Calculate KM for the substrate SAD.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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7. An enzyme is discovered that catalyzes the chemical reaction :
SAD
+ HAPPY
A team of motivated researchers sets out to study the enzyme, which they call happyase.
They find that the kcat for happyase is 600 s -1 and carry out several additional
experiments. When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity is 9.6 µM s-1.
Calculate KM for the substrate SAD.
8. In a separate enzyme happyase experiment using [Et] = 10 nM, the reaction velocity is
measured at 3 µM s-l. What is the [S] used in this experiment?
Transcribed Image Text:7. An enzyme is discovered that catalyzes the chemical reaction : SAD + HAPPY A team of motivated researchers sets out to study the enzyme, which they call happyase. They find that the kcat for happyase is 600 s -1 and carry out several additional experiments. When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity is 9.6 µM s-1. Calculate KM for the substrate SAD. 8. In a separate enzyme happyase experiment using [Et] = 10 nM, the reaction velocity is measured at 3 µM s-l. What is the [S] used in this experiment?
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