Calculate how to make 100 ml of “Alkaline Phosphatase Reaction Buffer”. You have on the shelf MgCl2 solid powder, 5M NaCl, 1M Tris @ pH 9.5, and 20% Tween-20. The final concentrations will be 5mM MgCl2, 0.1M NaCl, 0.1M Tris @ pH 9.5, and 0.3% Tween-20.

Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 15.BCP
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Calculate how to make 100 ml of “Alkaline Phosphatase Reaction Buffer”. You have on the shelf MgCl2 solid powder, 5M NaCl, 1M Tris @ pH 9.5, and 20% Tween-20. The final concentrations will be 5mM MgCl2, 0.1M NaCl, 0.1M Tris @ pH 9.5, and 0.3% Tween-20.

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