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Briefly explain the term cardinal temperatures and apply it to the terms psychrotrophic and mesophilic microorganism.
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- Which has a greater impact on the survival of an organism, temperatures above its maximum temperature or temperatures below its minimum temperature? Explain why and how this relates to typical methods used to control microbial growth.Compare and contrast oxygen requirements for obligate anaerobes and microaerophiles. Describe one laboratory media that can cultivate microbes with various oxygen requirements. Include the chemistry and how obligate anaerobes and microaerophiles would look if grown on this media.Describe the five phases of a microbial growth curve observed when growing microbes are grown in broth culture and discuss the causes of each
- Contrast an aerotolerant and an obligate anaerobe in terms of sensitivity to O2 and ability to grow in the presence of O2.List 5 physical methods of controlling microbial growth, and give an example of each. Why is moist heat much more effective than dry air?Define microbial growth. Outline the effects of temperature, pH, osmotic pressure and oxygen onmicrobial growth.
- TOPIC : MICROBIAL GROWTH Classify the bacteria that is most likely involved in each situation provided according to their classification (as to microbial growth requirements). CHOICES: A. Obligate aerobe ,B. Obligate anaerobe, C. Microaerophile, D. Aerotolerant anaerobe, E. Mesophile, F. Thermophiles 1.The bacteria under study thrives at the alveolar ducts in the lungs causing respiratory disease. 2.In the experiment, the agar plate containing an axenic culture was originally incubated inside a candle jar for one day and then transferred to an oxygen-rich incubator for another day. Findings: the microbial growth persisted and stayed the same regardless of the specific type of incubation employed. 3.A microorganism that can survive pickling and refrigeration was discovered when a tightly-sealed jar of pickles grew several colonies despite following the correct recipe, tight closure seal, and proper refrigeration. 4.The spoiled bottle of wine that was stored at standard room temperature…Compare and contrast the growth and physiology of obligate aerobes, facultative anaerobes, and strict anaerobesOxygen is always beneficial to micro-organisms. Discuss this statement in the context of factors that influence microbial growth.
- 1(a)What is a psychrotroph? (b)From what natural sources would you isolate a thermophile? A psychrophile? (C)How does temperature affect the growth of a microorganism? (D)State the temperature class for Escherichia coli, Bacillus sp, Aeromonas sp, Micrococcus luteus, and suggest their optimum growth temperature. 2 (a)Why is dilution important when determining microbe number? (B)How does a decrease in dye colour intensity affect the microbe ? (C)State the possible sources of error if plate counts and colour intensity of dilutions are incorrect or Precautions taken to prevent this from happening. ( this is not a graded assignment)Describe several physical and chemical requirements for microbial growth, and explain what factors determine optimal conditions.Staphylococcus aureus is non-motile, a facultative anaerobe, and a mesophile. At 37⁰C in abundant glucose, which incubation environment is more likely to produce the highest cell mass of S. aureus within two hours? Flask with aeration to add oxygen to the broth and shaking Flask left on the lab bench Flask grown in an anaerobic chamber that removes oxygen from the air around the flask Flask without aeration All will be equal