Biuret reagent used in protein assay consists of I. Biuret II. BSA III. Copper (II) sulfate IV. Strong alkaline A. I only B. III and IV C. All except II D. All except IV
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Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
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- Which of the following statements is/are FALSE about Bradford assay? 1. Upon addition of the reagent, the color of the protein solution becomes purple. II. The absorbance of the resulting solution is measured at a wavelength of 540-550 nm. Both I and II O II only Neither I nor II O I onlyB ut of 1 e flag us page In Canada, facilities that participate in sterile compounding follow guidelines provided by USP Chapter , NAPRA and this professional association: Select one: O a. CAPT Q b. CPTEA O C. FDA O d. CSHP FS Q Search F6 - F7 F8 L F9 m |--- ■■■ 8 + Time left 21:19: F10 Next pageBradford Assay: Coomassie Blue G-250, phosphoric acid: A. Biuret Protein Assay: sodium potassium tartrate, sodium carbonate, sodium hydroxide, copper (II) sulfate, Folin-Ciocalteau B. Lowry Assay: sodium hydroxide, copper (II) sulfate, sodium potassium tartrate. C. Bicinchoninic Acid Assay: bicinchoninic acid, sodium carbonate, sodium bicarbonate, sodium potassium tartrate, copper (II) sulfate D. Both A and B E. All of the above
- 60) An investigator is studying the immune response to an encapsulated strain of staphyloccoccus aureus. Two bacterila culture tubes are treated with human serum. Culture X contains a patient with a genetic deficiency in mannose bisning protein. Culturte Y contains serum form from a healthy individual. After 1 hour. bacterial lysis in culture Yis greater than thqatobtained in culture X. The investigator hypothesizes that the mannose bidning lectin activatesimmune system componentswith serum lysis of the bacteria. Which of the following system complements most likely performs this functions? (A) Complement (B) Eosinophil major basic protein (C) IgM (D) Lysozyme (E) Perforin (F) Toll-like receptor1. Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.2. Match the following molecules with the-corresponding test reagents that are used to identify them. Also, indicate the positive test color and what reagent component reacts with what specific chemical group that is responsible for a + test result Protein D Starch C Amino Acids B Sugar G Lipids A DNA F RNA E A. Sudan IV B. Ninhydrin C. Iodine D. Biuret E. Orcinol F. Diphenylamine G. Benedict
- Chemical tests See bottom of page 8 for instructions on how to complete this table. C. Biuret test Solution turins olive greeni Yeu ight Pur ple A. Iodine test B. Benedict's test Description of Solution turne Slohtion turns positive result Tube No. and (copy from Contents dark Purple or brovniornd asbiutrldeins Pp. 7-8) decp blue-blakk or tomoto of Short +/- Appearance blue (from p. 9) らish | +/- | Appearance blae Appearance +/- 1 - Orense 2 - Glucose Starch orunde Orange 3 - ders Brtelt blue blue blve bue 4 - alanine oransC- blue - 5. blue iout bue t orange Why was it important to have tube number 1 in each test? (Don't just say "because it's the control", and don't copy from the lab instructions-write out your own understanding of why and how that tube was used.)24. Arrange the correct sequence in southern blotting. I. DNA extracted from white cells and are cleaved. II. hybridization with p-labeled probe. III. Agarose gel electrophoresis. IV. Blotting technique V. Expose of x-ray film. O A. I, III, II, V, IV ) B.I, II, III, IV, V. OC.I, IV, III, V, II D. I, III, IV, II, V. Clear selection O O O1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.
- What staining is used for this microspecimen? a. Methilen blu b. Toluidin blu c. Eosinophilin d. Eosin e. Hematoxilin O f. PAS-ABG When is it appropriate for gloves X + rface/defaultui/player/modern.html?configuration=&preventRightClick=False&cc=en-US&ieCompatibilityMod... 2 Exit rientation to Infection Prevention Which of the following are proper disinfection steps? Check all the apply. O Remove organic matter O Disinfect only O Follow suggested contact time O Mix cleaning products to make the disinfectant more powerful O Disinfect O Disregard the product labels SUBMIT ENG INWhich of the following is a reason to run simultaneous tests on known positive and negative controls when identifying an unknown microbe using biochemical tests? O The controls maintain the differential qualities of the test medium The controls will ensure that the unknown microbe multiplies on the medium O The controls can show if there was contamination of the tubes with another species O The controls can show if there was a recent mutation in your unknown microbe MacBook Air DII F12 80 888 F7 F5 F4 F3 * & %2$ %23 6 7 8. 4 5