EXPERIMENT :UTILIZATION OF SOLID WASTE FOR PRODUCTION OF VALUE-ADDED PRODUCTS OBJECTIVES : To apply techniques/ technologies in waste management system for production of value-added products PROCEDURES: Separate the peels of different fruits were separated and oven dried at 50°C, ground and sieved through mesh screen. 50 ml of 10% (w/v) HCl was added to each waste (40 gm) in conical flask respectively. The mixture in water bath was placed at 100° C for one hour. The mixture was cooled down and the mixture was filtered through Whatman filter paper. The filtrate was diluted with sterile distilled water at varying concentration. The fruit hydrolysate was autoclaved at 121°C for 15 minutes. Saccharomyces cerevisiae was maintained on the YDPA at 28 °C. In 250 mL conical flasks, each of 100 mL of various fruit hydrolysate growth media supplemented was added with (NH4)2SO4 (2 gm), KH2PO4 (1gm), MgSO4.7H2O (0.5 gm), NaCl (0.1 gm), CaCl2 (0.1 gm). The initial pH of all the culture media was adjusted using H2SO4 and/or NaOH. Each of the medium (100 ml) was transferred into 250 ml Erlenmeyer flask and autoclaved 121°C for 15 minutes. 1 ml of Saccharomyces cerevisiae suspension was inoculated into each of the medium under sterile condition. The fermentation was carried at 28 °C under static condition for 6 days of incubation. After the 6th day of incubation, fermentation biomass was separated from the culture using vacuum filtration and washed with sterile water. The fermentation biomass was transferred into an aluminium disk and oven dried at 105 °C for one hour followed by cooling process in desiccators. The dry weight was measured and the moisture content of the SCP. QUESTION: Please explain briefly about how the procedures helps the experiment to succcess.

8th Edition

ISBN:9781259696527

Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart

Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart

Chapter1: Introduction

Section: Chapter Questions

Problem 1.1P

Related questions

Question

EXPERIMENT :UTILIZATION OF SOLID WASTE FOR PRODUCTION OF VALUE-ADDED PRODUCTS

OBJECTIVES :

To apply techniques/ technologies in waste management system for production of value-added products

PROCEDURES:

Separate the peels of different fruits were separated and oven dried at 50°C, ground and sieved through mesh screen.
50 ml of 10% (w/v) HCl was added to each waste (40 gm) in conical flask respectively.
The mixture in water bath was placed at 100° C for one hour. The mixture was cooled down and the mixture was filtered through Whatman filter paper. The filtrate was diluted with sterile distilled water at varying concentration. The fruit hydrolysate was autoclaved at 121°C for 15 minutes.
Saccharomyces cerevisiae was maintained on the YDPA at 28 °C.
In 250 mL conical flasks, each of 100 mL of various fruit hydrolysate growth media supplemented was added with (NH4)2SO4 (2 gm), KH2PO4 (1gm), MgSO4.7H2O (0.5 gm), NaCl (0.1 gm), CaCl2 (0.1 gm). The initial pH of all the culture media was adjusted using H2SO4 and/or NaOH.
Each of the medium (100 ml) was transferred into 250 ml Erlenmeyer flask and autoclaved 121°C for 15 minutes.
1 ml of Saccharomyces cerevisiae suspension was inoculated into each of the medium under sterile condition. The fermentation was carried at 28 °C under static condition for 6 days of incubation.
After the 6th day of incubation, fermentation biomass was separated from the culture using vacuum filtration and washed with sterile water. The fermentation biomass was transferred into an aluminium disk and oven dried at 105 °C for one hour followed by cooling process in desiccators.
The dry weight was measured and the moisture content of the SCP.

QUESTION:

Please explain briefly about how the procedures helps the experiment to succcess.

Expert Solution