Are there ways to reveal the detailedworkings of a protein machine that donot require the purification of each ofits component parts in large amounts,so that the machine’s functions canbe reconstituted and dissected usingchemical techniques in a test tube?

Q: Is it reasonable to expect that protein degradation can take place at any location in a cell?

A: Protein degradation is considered as the most important step in the cell, during which proteins are…

Q: What physical parameters of a protein control its migration on electrophoresis?

A: Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules in the…

Q: 39. The most expensive method to purify the protein is: Affinity Size exclusion RP-HPLC (reversed…

A: Proteins: Proteins are chemical substances that are made up of building blocks that are commonly…

Q: describe protein stuctures, prmary throgh quaternary and protein denaturation

A: According to central dogma, the final synthesis in body is from the RNA to protein. As the message…

Q: When will scientists be able totake any amino acid sequence andaccurately predict both that…

A: Amino acids are biomolecules consisting primarily of elements like carbon, hydrogen, oxygen, and…

Q: 1)What is the objective of hydrolyzing the RNA prior to doing biochemical tests? 2) What particular…

A: Asked : Objective of RNA hydrolysis  Test for pentose except Molisch's  RNA and DNA questions are…

Q: Two-dimensional electrophoresis usually exploits which 2 different properties of proteins?

A: Electrophoresis is a technology used in the lab in order to seperate and analyse sample of interest…

Q: a) What was the full peptide sequence before degradation? b) Paula thought she forgot to treat her…

A: Edmans degradation is a method of sequencing the amino acids in the peptide. In this first step…

Q: Discuss the general structure of the carrier proteins within our cells. Describe how their structure…

A: Integral membrane proteins called carrier proteins make it easier for molecules to penetrate cell…

Q: Can someone give me examples of denatured proteins that can be brought back to native state…

A: The question asks the examples of denatured protein which can be transformed into the renatured or…

Q: Describe shortly the process of Edman degradation to determine amino acid sequence in protein…

A: Proteins are an essential component of the body. In terms of molecular biology, it is the form of…

Q: 7. A protein has a molecular mass of 400 kDa when measured by gel filtration. When subjected to gel…

A: As we know that the sodium dodecyl (SDS) sulfate is considered as the denaturing agent, which helps…

Q: Experimentally, what are some ways to investigate a protein's function/s or loss of it?

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: protein purification through hydrophobic column chromatography

A: In hydrophobic interaction chromatography (HIC), molecules are separated based on how hydrophobic…

Q: Briefly describe the conditions of protein sample labeling and NMR sample. ?

A: Nuclear magnetic resonance or NMR is used to analyze the structure of a material. It analyzes the…

Q: What are other substances/situations that can cause protein precipitation?

A: Introduction: Proteins are nitrogenous macromolecules that are the polymers of amino acids. It…

Q: Why is denaturing the proteins necessary for SDS PAGE to work? Use YOUR OWN BERITEL

A: Introduction  Proteins are large, complex molecules that perform a wide range of essential functions…

Q: What is specific activity and how is it useful in protein purification'

A: Proteins are made up of amino acids linked by peptide bonds. Pure  form of proteins can be obtained…

Q: Please explain machanism of protein folding, highlighting the role of chaperones.

A: All cellular systems are controlled by proteins. The instruction for the synthesis of proteins is…

Q: How many uL of RNA would you add to a tube if you need 2ug and your RNA concentration is 333ug/mL?

A: RNA: A polymeric molecule called ribonucleic acid (RNA) is crucial for many biological processes,…

Q: What is the difference between an autonomous and a nonautonomoustransposable element? Is it possible…

A: Transposable elements (TEs) are the sequences of DNA (Deoxyribonucleic acid)  that can move from a…

Q: How might you separate two proteins by extraction if they had pka's of 5 and 7, respectivley?

A: Affinity chromatography is a separation technique based on a specific binding interaction between an…

Q: How do you separate proteins using SDS-PAGE?

A: Protein is a class of biomolecules that are regarded as the most essential component of life in…

Q: Why are integral proteins more difficult to study then peripheral, and why integral can only be…

A: The ease with which a molecule can flow through a cell membrane is referred to as "permeability".…

Q: What are the requirements of a laboratory dedicated to animal cell culture experiments?

A: Hello. Since you have posted multiple questions and not specified which question needs to be solved,…

Q: 1. The amino acid sequence for the protein lysozyme is given below. Estimate the isoelectric point…

A: Isoelectric point is the pH where the net charge on a protein is zero.

Q: Can you recommend the most suitable method for purification of peptides in acidic solution…

A: Introduction: Peptides are the short chain of amino acids connected by peptide bonds. It consists of…

Q: 14. A protein mixture consisting of proteins A, B, and C was subjected to various protein…

A: The objective of the question is to determine the properties of proteins A, B, and C based on their…

Q: Given the following information, which protein would be most difficult to isolate from protein X…

A: Salting out is a method of separation of protein mixtures that is based on protein solubility. The…