Benesi-Hildebrand Plot.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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![Create a Benesi-Hildebrand Plot. To do this, plot the data of 1/(F-F0) (y-axis) vs.
1
X-
(F-F0) Ka(Foo-F0) [CD]° (Foo-F0)
1
1/[CD]0 (x-axis). Fit the data to Equation
The fitting parameters of the data to a straight-line equation (y = mx + b)
should allow you to calculate Ka. Take care with units, concentrations will need to be M when reporting
the Ka value.
[ME-b-CD]
X ul of ME-b-CD
ul of ANS
Y ul of
Fluorescence
(mM)
(20mM)
(0.4mM)
PBS
[CD] mM
intensity
F- FO
0.
500
1500
0.
0.37
1
100
500
1400
9.426
9.056
200
500
1300
2.
16.76
16.39
3
300
500
1200
3.
22.621
22.251
4.
400
500
1100
4.
27.496
27.126
.500
500
1000
5.
30.573
30.203
6.
600
500
900
6.
34.344
33.974
700
500
800
7.
36.991
36.621
8.
800
500
700
8.
38.478
38.108
900
500
600
9.
41.012
40.642
10
1000
500
500
10
43.769
43.399
12
1200
500
300
12
45.64
45.27
14
1400
500
100
14
47.52
47.15
15
1500
500
15
48.359
47.989](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fed3ef329-d408-459b-9abf-7e1a6ce4fb0d%2F944567f2-46cb-43b8-964d-6790c4119ba7%2Fndaggeg_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Create a Benesi-Hildebrand Plot. To do this, plot the data of 1/(F-F0) (y-axis) vs.
1
X-
(F-F0) Ka(Foo-F0) [CD]° (Foo-F0)
1
1/[CD]0 (x-axis). Fit the data to Equation
The fitting parameters of the data to a straight-line equation (y = mx + b)
should allow you to calculate Ka. Take care with units, concentrations will need to be M when reporting
the Ka value.
[ME-b-CD]
X ul of ME-b-CD
ul of ANS
Y ul of
Fluorescence
(mM)
(20mM)
(0.4mM)
PBS
[CD] mM
intensity
F- FO
0.
500
1500
0.
0.37
1
100
500
1400
9.426
9.056
200
500
1300
2.
16.76
16.39
3
300
500
1200
3.
22.621
22.251
4.
400
500
1100
4.
27.496
27.126
.500
500
1000
5.
30.573
30.203
6.
600
500
900
6.
34.344
33.974
700
500
800
7.
36.991
36.621
8.
800
500
700
8.
38.478
38.108
900
500
600
9.
41.012
40.642
10
1000
500
500
10
43.769
43.399
12
1200
500
300
12
45.64
45.27
14
1400
500
100
14
47.52
47.15
15
1500
500
15
48.359
47.989
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