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Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the concentration of BSA in each sample and say if the standard curve is adequate for each of these samples? Why or why not? If not, what would you do to fix this? Show your

Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the concentration of BSA in each sample and say if the standard curve is adequate for each of these samples? Why or why not? If not, what would you do to fix this? Show your

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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help please with calculations

Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the concentration of BSA in each sample and say if the standard curve is adequate for each of these samples? Why or why not? If not, what would you do to fix this? Show your calculations. Absorbance 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 y = 0.9825x - 0.1079 R² = 0.991 0.2 0.1 0.2 0.4 0.6 0.8 1 1.2 BSA concentration % (m/v) Curve equation: y=0.9825x-0.1079 R?=0.991 Absorbance Sample Reading 1 Reading 2 Sample A 0.578 0.536 Sample B Sample C 0.966 1.042 0.184 0.203 Absorbance
Transcribed Image Text:Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the concentration of BSA in each sample and say if the standard curve is adequate for each of these samples? Why or why not? If not, what would you do to fix this? Show your calculations. Absorbance 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 y = 0.9825x - 0.1079 R² = 0.991 0.2 0.1 0.2 0.4 0.6 0.8 1 1.2 BSA concentration % (m/v) Curve equation: y=0.9825x-0.1079 R?=0.991 Absorbance Sample Reading 1 Reading 2 Sample A 0.578 0.536 Sample B Sample C 0.966 1.042 0.184 0.203 Absorbance
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