Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and oxygen [Chem. Eng. Educ., 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C. t (min) 0 10 20 50 Cuyo, (mol/L) 0.02 0.01775 0.0158 0.0106 100 0.005 (a) Determine the Michaelis-Menten parameters Vax and KM- (b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20 minutes?
Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and oxygen [Chem. Eng. Educ., 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C. t (min) 0 10 20 50 Cuyo, (mol/L) 0.02 0.01775 0.0158 0.0106 100 0.005 (a) Determine the Michaelis-Menten parameters Vax and KM- (b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20 minutes?
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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![Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and
oxygen [Chem. Eng. Educ., 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function
of time for a reaction mixture with a pH of 6.76 maintained at 30°C.
t (min)
0
10
20
50
100
C₁,0, (mol/L) 0.02 0.01775 0.0158 0.0106 0.005
(a) Determine the Michaelis-Menten parameters Vmax and KM-
(b) If the total enzyme concentration is tripled, what will the substrate concentration be after
20 minutes?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Faac0f6cd-6ab1-4fb9-8b49-ef0c225cb05e%2F753aea13-5fbe-45a9-9d50-46c408819354%2F9d723y9_processed.png&w=3840&q=75)
Transcribed Image Text:Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and
oxygen [Chem. Eng. Educ., 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function
of time for a reaction mixture with a pH of 6.76 maintained at 30°C.
t (min)
0
10
20
50
100
C₁,0, (mol/L) 0.02 0.01775 0.0158 0.0106 0.005
(a) Determine the Michaelis-Menten parameters Vmax and KM-
(b) If the total enzyme concentration is tripled, what will the substrate concentration be after
20 minutes?
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