Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter15: Acid–base Equilibria
Section: Chapter Questions
Problem 99AP
Related questions
Question
Part B only
![LC analysis are sometime performed using buffers as mobile phase to control the pH. The following table lists the
retention factors (k) for three analytes separated on a C8 non-
polar column. The mobile phase was a citrate buffer: methanol
mixture adjusted to given pHs with NH3.
a. With given structures of acetophenone, nicotine,
and salicylic acid, fill out the following table with
appropriate major species at each pH.
Structure
Acetophenone (no
pka)
Nicotine
(pka1 = 3.15,
pka2 = 7.85)
Salicylic acid
(pka1 = 2.97,
pKa2 = 13.60)
CH3
CH3
OH
OH
pH 2
-CH3
CHS
OH
Ha
Analyte
Acetophenone
Nicotine
Salicylic Acid
pH 5
pH 2
8.5
0.0
6.0
ott
CH3
CHS
jó
pH 5
8.5
0.2
1.1
pH 8
CH3
N
pH 8
8.5
6.1
1.1
CH3
Of
b. Based on your structures above, provide explanations on why some of the observed retention factors
change with pH of the mobile phase.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0ee67c44-0754-41fd-9006-bdc882dbaccb%2Fd5b76c31-24d9-48f7-9861-77e40612c48f%2Fgz0mmui.jpeg&w=3840&q=75)
Transcribed Image Text:LC analysis are sometime performed using buffers as mobile phase to control the pH. The following table lists the
retention factors (k) for three analytes separated on a C8 non-
polar column. The mobile phase was a citrate buffer: methanol
mixture adjusted to given pHs with NH3.
a. With given structures of acetophenone, nicotine,
and salicylic acid, fill out the following table with
appropriate major species at each pH.
Structure
Acetophenone (no
pka)
Nicotine
(pka1 = 3.15,
pka2 = 7.85)
Salicylic acid
(pka1 = 2.97,
pKa2 = 13.60)
CH3
CH3
OH
OH
pH 2
-CH3
CHS
OH
Ha
Analyte
Acetophenone
Nicotine
Salicylic Acid
pH 5
pH 2
8.5
0.0
6.0
ott
CH3
CHS
jó
pH 5
8.5
0.2
1.1
pH 8
CH3
N
pH 8
8.5
6.1
1.1
CH3
Of
b. Based on your structures above, provide explanations on why some of the observed retention factors
change with pH of the mobile phase.
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