Based on the relative increase in purity, place the purification procedures used for this enzyme in order of effectiveness. Give the most effective procedure first, that is, the one that gives the greatest relative increase in purity.
Based on the relative increase in purity, place the purification procedures used for this enzyme in order of effectiveness. Give the most effective procedure first, that is, the one that gives the greatest relative increase in purity.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Based on the relative increase in purity, place the purification procedures used for this enzyme in order of effectiveness. Give the
most effective procedure first, that is, the one that gives the greatest relative increase in purity.
Most effective
Least effective
Answer Bank
ion-exchange chromatography
precipitation (pH)
precipitation (salt)
affinity chromatography
size-exclusion chromatography
Transcribed Image Text:A biochemist discovers and purifies a new enzyme, generating the purification table shown.
From the information given, complete the table column showing the specific activity of the enzyme after each
purification procedure.
Total protein
Total activity
Specific activity
(units mg-)
Procedure
(mg)
(units)
Crude extract
10, 000
68, 000
Precipitation (salt)
5, 000
65, 000
Precipitation (pH)
4, 000
56, 000
Ion-exchange chromatography
70
49, 000
Affinity chromatography
12
42, 000
Size-exclusion chromatography
8
40, 000
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