BAPNA ΒΑΡΝΑ Abs @410 [p-nitroanil [p-nitroanil nm (mm) 0 0.2625 0.525 1.05 2.1 4.2 (mm) 0 0.0656 0.1312 0.2625 0.525 1.05 0 0.495 0.416 0.526 0.655 0.653 ine] (M) ine] (nmol) 0 0 0.00005625 62.5 0.00004727 47.27 0.00005977 59.77 0.00007443 74.43 0.0000742 74.2 [p-nitroanil ine] (nmol/min) 0 6.25 4.727 5.977 7.443 7.42 1/S (mm) (nmol/ -1 mi n) n/a n/a 15.24 0.16 7.62 3.80 1.90 1/V 0.95 0.211 0.167 0.134 0.134
BAPNA ΒΑΡΝΑ Abs @410 [p-nitroanil [p-nitroanil nm (mm) 0 0.2625 0.525 1.05 2.1 4.2 (mm) 0 0.0656 0.1312 0.2625 0.525 1.05 0 0.495 0.416 0.526 0.655 0.653 ine] (M) ine] (nmol) 0 0 0.00005625 62.5 0.00004727 47.27 0.00005977 59.77 0.00007443 74.43 0.0000742 74.2 [p-nitroanil ine] (nmol/min) 0 6.25 4.727 5.977 7.443 7.42 1/S (mm) (nmol/ -1 mi n) n/a n/a 15.24 0.16 7.62 3.80 1.90 1/V 0.95 0.211 0.167 0.134 0.134
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Can you graph the data
![ΒΑΡΝΑ
(mm)
0
0.2625
0.525
1.05
2.1
4.2
ΒΑΡΝΑ Abs @410 [p-nitroanil [p-nitroanil [p-nitroanil 1/S 1/V
nm
ine]
(nmol/min)
(mm)
0
0.0656
0.1312
0.2625
0.525
1.05
0
0.495
0.416
0.526
0.655
0.653
ine]
(M)
0
0
0.00005625 62.5
ine]
(nmol)
0.00004727 47.27
0.00005977 59.77
0.00007443 74.43
0.0000742
74.2
0
6.25
4.727
5.977
7.443
7.42
(mM) (nmol/
mi n)
n/a
0.16
n/a
15.24
7.62
3.80
1.90
0.95
0.211
0.167
0.134
0.134](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc86cabf0-1cfc-4015-b001-d798801597af%2Fae9b265d-8081-4e7d-b85c-ef4d387d2756%2Fto52fwg_processed.jpeg&w=3840&q=75)
Transcribed Image Text:ΒΑΡΝΑ
(mm)
0
0.2625
0.525
1.05
2.1
4.2
ΒΑΡΝΑ Abs @410 [p-nitroanil [p-nitroanil [p-nitroanil 1/S 1/V
nm
ine]
(nmol/min)
(mm)
0
0.0656
0.1312
0.2625
0.525
1.05
0
0.495
0.416
0.526
0.655
0.653
ine]
(M)
0
0
0.00005625 62.5
ine]
(nmol)
0.00004727 47.27
0.00005977 59.77
0.00007443 74.43
0.0000742
74.2
0
6.25
4.727
5.977
7.443
7.42
(mM) (nmol/
mi n)
n/a
0.16
n/a
15.24
7.62
3.80
1.90
0.95
0.211
0.167
0.134
0.134
![a) The Michaelis-Menten plot
Using the graph paper provided, plot the velocity of the reaction (v) against the concentration
of BAPNA substrate ([S]). Fit a curve to the data and estimate the Vmax and Km.
How would you describe the relationship between the amount of product formed and the
substrate concentration? For example, would you say that it is a linear, hyperbolic or
exponential relationship?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc86cabf0-1cfc-4015-b001-d798801597af%2Fae9b265d-8081-4e7d-b85c-ef4d387d2756%2Fgdtl27c_processed.jpeg&w=3840&q=75)
Transcribed Image Text:a) The Michaelis-Menten plot
Using the graph paper provided, plot the velocity of the reaction (v) against the concentration
of BAPNA substrate ([S]). Fit a curve to the data and estimate the Vmax and Km.
How would you describe the relationship between the amount of product formed and the
substrate concentration? For example, would you say that it is a linear, hyperbolic or
exponential relationship?
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