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INTRODUCTION
Biuret test
It is a chemical test used to detect the presence of proteins.
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- C. Answer the following questions: 8. Why does the methylene blue dye stain the nuclei in onion cells? 9. What is the cause of the variation in color intensity in a biochemical test? 10. You have negative results on both the Benedict and Biuret tests but both look blue. Why?C. Humulin R drip 1 unit/ml in 50 ml NSS. Humulin R is available as 100 units per ml, 10 ml vials. How many ml of Humulin R is needed to prepare the solution?B. Why do we wash the Ni-NTA beads after applying the clarified lysate so many times? What would happen if you skipped the wash steps?
- Identify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or class of lipids that are detected using these methods.a. How much NS was diluted? b. How much Gentamicin was diluted? c what is the desired concentration?State the principle of the antiglobulin test. Differentiate monoclonal from polyclonal and monospecific from polyspecific antihuman globulin (AHG) reagents.
- Identify and name the disease in which the patient’s cells lose the property of contact inhibition. State its possible causes and explain any three methods to accurately detect the pathological and physiological changes that take place due to the disease in living tissues.introduction Background information about the TLC technique ,should include: a- The phases. b- The principle of the technique. 2- Basic structure of amino acids. 3- Definition of the Retention Factor (Rf). aim :A. Prepare a diagram with labels showing the similarities and differences among the four major types of ELISA based on their capture and detection systems. A1. Give at least one clinical application for each type of ELISA. B. What is the purpose of adding horseradish peroxidase, alkaline phosphatase, and their respective substrates?
- Nursing question [3. make table to differentiate ITP, TTP, HIT, DIC.A. Differentiate the chemistry and function of primary and secondary antibodies in ELISA. B. Discuss the purpose of adding a blocking buffer. What happens when you fail to add the blocking buffer? Why do we use BSA as the primary component of the blocking buffer?In this age of COVID-19, hand washing is very important. Describe the nature of a surfactant, and how detergents are chemically designed to help clean hands.