The answer to part a is -22.4I need help with part b thank you ATP is synthesized from ADP, P, and a proton on the matrix side of the inner mitochondrial membrane.We will refer to the matrix side as the "inside" of the Inner Mitochondrial Membrane (the "IMM"). Show yourwork.(a) H* transport from the outside of the IMM into the matrix drives this process. Assume the pH insidethe matrix is 8.00 and the outside is more acidic by 0.60 pH units. Assuming the IMM membranepotential is 165.0 mV (inside negative), calculate AG for the transport of1 mol of H* across the IMM into the matrix at 37 °C: H* (outside) → H*(inside)(b) Assume four mol H* must be translocated to synthesize one mol ATP by coupling of the followingreactions:ADP + P + H* (inside) → ATP + H₂O4 H* (outside) → 4 H* (inside)(ATP synthesis)(proton transport)Given the following steady-state concentrations: ATP = 4.10 mM, ADP = 65.0 μM and P₁ = 5.30 mM, themembrane potential = 165.0 mV (inside negative), and the pH values in part (a), calculate AG at 37 °Cfor the synthesis of ATP.

If the three moles of translocate to synthesize one mole of ATP, then coupled reaction is as…

(b) Assume four mol H* must be translocated to synthesize one mol ATP by coupling of the following reactions: ADP + P +H* (Inside) → ATP + H₂O 4 H* (outside) →4 H*(inside) (ATP synthesis) (proton transport) Given the following steady-state concentrations: ATP = 4.10 mM, ADP = 65.0 μM and P₁ = 5.30 mM, the membrane potential = 165.0 mV (inside negative), and the pH values in part (a), calculate AG at 37 °C for the synthesis of ATP.

(b) Assume four mol H* must be translocated to synthesize one mol ATP by coupling of the following reactions: ADP + P +H* (Inside) → ATP + H₂O 4 H* (outside) →4 H*(inside) (ATP synthesis) (proton transport) Given the following steady-state concentrations: ATP = 4.10 mM, ADP = 65.0 μM and P₁ = 5.30 mM, the membrane potential = 165.0 mV (inside negative), and the pH values in part (a), calculate AG at 37 °C for the synthesis of ATP.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

ATP is synthesized from ADP, P, and a proton on the matrix side of the in- ner mitochondrial membrane. We will refer to the matrix side as the "inside" of the inner mitochondrial membrane (IMM). (a) H* transport from the outside of the IMM into the matrix drives this process. The pH inside the matrix is 8.2, and the outside is more acidic by 0.8 pH units. Assuming the IMM membrane potential is 168 mV (inside negative), calculate AG for the transport of 1 mol of H* across the IMM into the matrix at 37 °C: Houtside) Hinside) (b) Assume three mol H* must be translocated to synthesize one mol ATP by coupling of the following reactions: ADP + P, + Hinskde) ATP + H,O(ATP synthesis) 3Hinside)(proton transport) 3Houtside)
(A) What is the free energy yield associated with the transport of a proton from the mitochondrial intermembrane space (IMS) to the mitochondrial matrix, assuming that the pH of the IMS is 1.4 units lower than the pH of the matrix? (Hint: Calculate AGO' for the reaction H"IMS > H matris using the relationship AG° = RTInKeg and remembering that pH = -log1o[H*]. Assume that T = 310K (37°C), and R = 8.315x10-3 kJ mol-1 K-1. Show your work and include correct units!)
The difference in pH between the internal and external surfaces of the inner mitochondrial membrane is 1.4 pH units (external side acidic). If the membrane potential is 0.06 V (inside negative), what is the free energy change on transporting 1 mol of protons across the membrane from outside to inside at 25°C?
Imagine that in red blood cells (RBCS) the following concentrations were noted for the "adenylate pool": [ATP] = 2000 uM; [ADP] = 270 uM; and [AMP] = 30 uM. ASsume that the pH is 7 and remains constant. a) calculate the Energy change (EC) (to four decimal places for RBCS given the above values. Show work b) If the [ATP] decreases by 5%, calculate the new [ATP], [ADP], and [AMP] given that the concentrations of all three species are "connected" by the adenylate kinase reaction, which is shown below. You will need to use the quadratic equation for this one. show your work. %3| %3D ADP + ADP ATP + AMP Keq = 0.44
Assuming that the pH of the IMS is 1.4 units lower than the pH of the matrix, the free energy yield associated with the transport of a proton from the mitochondrial intermembrane space (IMS) to the mitochondrial matrix is △ G of the reaction is 8.310 KJmol-1 What is the minimum number of protons that must enter the mitochondrial membrane from the IMS in order to synthesize one molecule of ATP?
A 75 kg person doing light work requires about 3000 kcal of food energy perday, 40% of which is actually used by the body’s cells. (The other 60% is lostas heat and in waste products.) Before being used by the cells, effectively allof this energy is stored in ATP, which is then cleaved into ADP and PO3−2 , with the release of 12.5 kcal per mole of ATP.(a) How many moles of ATP are turned over per day in this fashion? Whatmass of ATP does this correspond to? (The molecular weight of ATP is507 g/mol.)(b) The body actually contains approximately 5 g ATP. Estimate the averagerecycle time for an ATP molecule. You see that it is much more efficientto reuse ADP rather than to synthesize it de novo (from scratch).
The parietal cells of the stomach lining contain membrane “pumps” that transport hydrogen ions from the cytosol (pH 7.0) into the stomach,contributing to the acidity of gastric juice (pH 1.0). Calculate the free energy required to transport 1 mol of hydrogen ions through these pumps. Assume a temperature of 37 °C.
Carbonic anhydrase catalyzes the hydration of CO. CO2 + H2O ¬ H½CO3 The Km of this enzyme for CO, is 1.20×104 µ.M. When [CO,] = 3.60×104 µM, the rate of reaction was 4.50 umol·mL! sec-1 a What is Vmax for this enzyme? umol·mL-!sec-!
According to Paul Boyer, the mechanism of ATP synthesis involves the rotation of the y subunit which in turn causes sequential conformation changes in the aß assemblies. Explain the steps of ATP synthesis according to this mechanism.
a) Assuming that ubiquinone is unavailable inside of the cell, calculate the AG and the Keq if electrons are transferred directly from complex I to complex III of the electron transport chain. In your answer include the net equation for this electron transfer. Constants: R= 8.3J/degree'mol, F= 96.1kJ/volt mol, T=298K ( Half Reaction EM ubiquinone + 2e + 2H' Ubiquinol + H2 NAD + 2e+ 2H'→ NADH + H 0.045 -0.320 1/202 + 2e +2H H,O Cytc (Fe") + +le + 1H"→ Cytc (Fe²) 0.816 0.254 b) In a single sentence explain whether or not this electron transfer is possible. /
During vigorous exercise, a buffer system consisting of lactic acid and sodium lactate is produced in the muscle cells and released into circulation. (a) Calculate the concentration of lactate in the blood plasma (pH= 7.40) if the concentration of lactic acid in the blood is 1.50µM. {The pKa for lactic acid is 4.76}. (b) How is NADH recycled to NAD+when oxygen is a limiting factor during vigorous exercise? (c) Indicate the biochemical transformation that occurs in (b) above when aerobic conditions are restored during the resting state.
Gastric juice (pH 1.5) is produced by pumping HCl from blood plasma (pH 7.4) into the stomach. Calculate the amount of free energy required to concentrate the H+ in 1 L of gastric juice at 37 °C. Under cellular conditions,how many moles of ATP must be hydrolyzed to provide this amount of free energy? The free-energy change for ATP hydrolysis under cellular conditions is about −58 kJ/mol . Ignore the effects of the transmembrane electrical potential.