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b) After generating fluorescent synaptic regions by this procedure, suppose that you trigger additional action potentials while continuing to wash with medium. Would you expect the synaptic regions to lose fluorescence? Explain.

b) After generating fluorescent synaptic regions by this procedure, suppose that you trigger additional action potentials while continuing to wash with medium. Would you expect the synaptic regions to lose fluorescence? Explain.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Evidence Question #7 1. Various types of vesicle coats have been implicated in membrane traffic pathways such as endocytosis, export from the ER to the Golgi, and transport between Golgi cisternae. But so far, no coat has been identified for the secretory vesicles that carry material from the Golgi to the plasma membrane. Do you think such a coat is likely to exist? Justify your answer. 2. A muscle cell contains acetylcholine receptors that act as ligand-gated cation channels, and it also contains voltage-gated Na+ channels of the type found in neurons. In theory, could a muscle cell get away with having only acetylcholine-gated cation channels, which would serve the dual purpose of receiving neurotransmitter signals and propagating action potentials? Why or why not? 3. Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish. You incubate the neurons with an FM dye, and then you wash them with medium to remove the dye. a) You find that if the neurons are stimulated to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect. b) After generating fluorescent synaptic regions by this procedure, suppose that you trigger additional action potentials while continuing to wash with medium. Would you expect the synaptic regions to lose fluorescence? Explain.
Transcribed Image Text:Evidence Question #7 1. Various types of vesicle coats have been implicated in membrane traffic pathways such as endocytosis, export from the ER to the Golgi, and transport between Golgi cisternae. But so far, no coat has been identified for the secretory vesicles that carry material from the Golgi to the plasma membrane. Do you think such a coat is likely to exist? Justify your answer. 2. A muscle cell contains acetylcholine receptors that act as ligand-gated cation channels, and it also contains voltage-gated Na+ channels of the type found in neurons. In theory, could a muscle cell get away with having only acetylcholine-gated cation channels, which would serve the dual purpose of receiving neurotransmitter signals and propagating action potentials? Why or why not? 3. Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish. You incubate the neurons with an FM dye, and then you wash them with medium to remove the dye. a) You find that if the neurons are stimulated to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect. b) After generating fluorescent synaptic regions by this procedure, suppose that you trigger additional action potentials while continuing to wash with medium. Would you expect the synaptic regions to lose fluorescence? Explain.
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