ATP, R =119? And y intercept
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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ATP, R =119? And y intercept
![The data in the table were collected for the myosin-catalyzed
hydrolysis of ATP. Use these data to determine the values of
[ATP] (µmol·L¯!)
R(pmol·L-.s-1)
Rmax (the maximum rate of the enzyme, often called the
7.5
67.0
maximum velocity, Vmax) and KM, the Michaelis–Menten
12.5
95.0
constant.
20.0
119
43.5
155
62.5
166
If you were to plot this data to graphically determine Rmax and KM using a Lineweaver–Burk plot, what would you plot for
the data? If you would plot the original data, enter the original value.
= 12.5 µmol·L-: graph as
L-pmol-
[ATP]
0.00000008
L-s-pmol-
.0105
R = 95.0 pmol·L-.s-: graph as
L-pmol-
0.000000125
[ATP]
= 20.0 µmol·L: graph as
Publisher: University Science Books
Question Source: MRG - General Chemistry
MacBook Pro'](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F53b3b21a-5125-4b2d-af95-8c7b3e85050d%2Fc3255076-dfa0-4d5d-a8da-7e3bd33c4c10%2Fx2wxn3g_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The data in the table were collected for the myosin-catalyzed
hydrolysis of ATP. Use these data to determine the values of
[ATP] (µmol·L¯!)
R(pmol·L-.s-1)
Rmax (the maximum rate of the enzyme, often called the
7.5
67.0
maximum velocity, Vmax) and KM, the Michaelis–Menten
12.5
95.0
constant.
20.0
119
43.5
155
62.5
166
If you were to plot this data to graphically determine Rmax and KM using a Lineweaver–Burk plot, what would you plot for
the data? If you would plot the original data, enter the original value.
= 12.5 µmol·L-: graph as
L-pmol-
[ATP]
0.00000008
L-s-pmol-
.0105
R = 95.0 pmol·L-.s-: graph as
L-pmol-
0.000000125
[ATP]
= 20.0 µmol·L: graph as
Publisher: University Science Books
Question Source: MRG - General Chemistry
MacBook Pro'
Transcribed Image Text:R = 119 pmol-L-.s: graph as
95.0
L-s-pmol-
Incorrect
Calculate the slope using the data points from the first part. Then, determine the y-intercept using the second point.
slope =
71040.17
Enter numeric value
y-intercept: 0.00840336
L-s-pmol-
Incorrect
Use the y-intercept to calculate Rmax -
pmol-L-.s-
Rmax =
217.39
Question Source: MRG - General Chemistry Publisher: University Science Bo
MacBook Pro'
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