ATP ADP Phosphoglucomutase Glucose 1-phosphate .................... Glycogen ATP ADP Fructose 6-phosphate PFK-1 Fructose 1,6-biphosphate Glucose Glucose Hexokinase 6-phosphate G6P Isomerase NADP+ NADPH 6-phosphogluconolactone G6P Dehydrogenase Nucleotides

How many ATP are produced from the complete degradation of G3P using glycerol- three phosphate shuttle?

In angiogenic endothelial cells, pyruvate is converted to lactate (generating 2 ATP per glucose) rather than being completely oxidized (which would generate ~32 ATP by oxidative phosphorylation). Explain why angiogenic cells generate ATP anaerobically.

The effect of ATP on the allosteric enzyme PFK-1 is shown below. For a given concentration of fructose 6-phosphate, the PFK-1 activity increases with increasing concentrations of ATP, but a point is reached beyond which increasing the concentration of ATP inhibits the enzyme.     (a) Explain how ATP can be both a substrate and an inhibitor of PFK-1. How is the enzyme regulated by ATP?   (b) In what ways is glycolysis regulated by ATP levels?   (c) The inhibition of PFK-1 by ATP is diminished when the ADP concentration is high, as shown in the illustration. How can this observation be explained?   *A graph is included for this question*

What is the rationale for the inhibition of muscle glycogen phosphorylase by glucose 6- phosphate when glucose 1- phosphate is the product of the phosphorylase reaction?

In muscle tissue, the rate of conversion of glycogen to glucose 6-phosphate is determined by the ratio of phosphorylase a (active) to phosphorylase b (less active). Determine what happens to the rate of glycogen breakdown if a muscle preparation containing glycogen phosphorylase is treated with: (a) phosphorylase kinase and ATP; (b) PP1; (c) epinephrine

Consider oleic acid (18:1∆9): 1. How many NADH will be produced from complete oxidation of this fatty acid? 2. How many FADH2 will be produced from complete oxidation of this fatty acid? 3. Total number of ATP produced from NADH in complete oxidation of oleic acid (exclude transport cost of the fatty acid)? 4. Total number of ATP produced from FADH2 in complete oxidation of oleic acid (exclude transport costs of the fatty acid)? Please provide how you got them.

How many protons are required to synthesize one ATP by F1F0-ATPase containing (a) 10 or (b) 15 c subunits?

2. (a) ( In contrast to the pyruvate dehydrogen- ase complex, the a-ketoglutarate dehydrogenase (aKGDH) complex is not up- or downregulated by phosphorylation or dephosphorylation. However, the complex exhibits cooperativity modulated by the presence of ADP, ATP, inorganic phosphate (Pi), and Ca2+, as illustrated by the diagram on the right for the bovine kidney enzyme complex. Note in the diagram how the addition of 10 μM Ca2+ shifts the affinity of the enzyme complex for aKG from 20 mM Pi/-Ca2+ to 20 mM Pi/+Ca2+. Calcium especially en- hances the cooperative influence of ADP and ATP. Using the expanded copy of the diagram at the end of the problem set, estimate the change in S0.5 (re- member that for allosteric enzymes S0.5 corresponds to KM of a nonallosteric enzyme) for the enzyme complex in the presence of 20 mM Pi/-Ca2+ and in the presence of 20 mM Pi/+Ca2+. Compare similarly the change in S0.5 for the enzyme in the presence of 20 mM Pi/-Ca2+ plus 1.6 mM ADP to the enzyme in the…

How much ATP is produced from the complete B-Oxidation of myristic acid (C14H2802)? Activation of Fatty Acid ACCOA x (10 ATP/ACCOA) FADH₂ x (1.5 ATP/FADH₂) = NADH x (2.5 ATP/NADH) : = = -2 ATP ATP ATP ATP TOTAL ATP C18 CH, (CH₂)₁4-CH₂-CH₂-C-S-COA || FAD →FADH₂ CH₂(CH₂)₁4-CH=CH-C-S-CoA Each loop of the pathway represents a repetition of Steps 1-4. H₂O OH CH₂(CH₂)₁4-CH-CH₂-C-S-CoA || - NAD+ →→NADH+ H 0 CH3-(CH₂) 14 C-CH₂-C-S-CoA 0 || C16 CH₂(CH₂)14-C-S-CoA COA-SH Acyl CoA trans-Enoyl COA L-B-Hydroxyacyl CoA B-Ketoacyl COA H₁-C-S- + CH₂-C-S-CoA Acetyl CoA New acyl COA C₁4+ Acetyl CoA C12+ Acetyl CoA CO+ Acetyl CoA Cg+ Acetyl CoA C + Acetyl COA C₁ + Acetyl COA 2 Acetyl COA