A key step in the biosynthesis of triglycerides (fats) is the conversion of glycerol to glycerol-1-phosphate by ATP:At a steady state in the living cell, (ATP) = 10-3M and (ADP) = 10-4 M. The maximum (equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 °Cand pH=7.Calculate K and DrG° for the reaction.Using the value of DrG° for the reaction ADP + Pi àATP + H2O, together with the answer to part (a), calculate DrG° and K for the reaction: glycerol + phosphateà glycerol-1-phosphate+ H2O . Mg2+a-glycerol kinaseglyserol-1-phosphate (G-1-P) + ADPGlyserol (G) +АТР

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A key step in the biosynthesis of triglycerides (fats) is the conversion of glycerol to glycerol-1-phosphate by ATP:

At a steady state in the living cell, (ATP) = 10^-3M and (ADP) = 10^-4 M. The maximum (equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 °C

and pH=7.

Calculate K and D_rG° for the reaction.
Using the value of D_rG° for the reaction ADP + P_i àATP + H₂O, together with the answer to part (a), calculate D_rG° and K for the reaction: glycerol + phosphateà glycerol-1-phosphate+ H₂O .

Mg2+
a-glycerol kinase
glyserol-1-phosphate (G-1-P) + ADP
Glyserol (G) +
АТР

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