Assuming the molar extinction coefficient for para-nitrophenyl phosphate (PNP) is55,290 mol "dm cm, calculate the concentration of the molecule that produced anabsorbance of 0.770 OD units in a 1.5 cm cuvette. Show all working-13-1

Given Values: ε=55290 c = ?A = 0.77 l = 1.5 cm

Answered: Assuming the molar extinction…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Calculate the extinction coeficcient where the concentration is in mg/ml and the path length is in cm. The molecular weight of A is 290 g/mol. Re calculate the extinction coefficient with the concentration in nM. Note the unit of the newly calculated extinction coefficient will contain a mM-1 term. Given: Concentration: 5 microgram path length: 1cm absorbance: 0.055
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Assuming the molar extinction coefficient for para-nitrophenyl phosphate (PNP) is 55,290 mol "dm cm¯, calculate the concentration of the molecule that produced an absorbance of 0.770 OD units in a 1.5 cm cuvette. Show all working -1 3 -1