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Treatment with Ellman's reagent (DTNB; (5,5')-dithig-bis-(2-nitrobenzoic acid; MW = 296.4 g/mol) produced an absorbance of A412 nm = 1.025. If the extinction coefficient for the thiolate form of Ellman's reagent = 14,100 M-1-cm-1 (which arises when Ellman's reagent reacts with a single Cys-SH group), then how many free cysteine residues (Cxs-SH) are in this protein? Sulfhydryl Molecule DTNB2- 0. R-S HS- -N Mixed Disulfide TNB2- シ=0

Treatment with Ellman's reagent (DTNB; (5,5')-dithig-bis-(2-nitrobenzoic acid; MW = 296.4 g/mol) produced an absorbance of A412 nm = 1.025. If the extinction coefficient for the thiolate form of Ellman's reagent = 14,100 M-1-cm-1 (which arises when Ellman's reagent reacts with a single Cys-SH group), then how many free cysteine residues (Cxs-SH) are in this protein? Sulfhydryl Molecule DTNB2- 0. R-S HS- -N Mixed Disulfide TNB2- シ=0

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Treatment with Ellman's reagent (DTNB; (5,5')-difthig-bis-(2-nitrobenzoic acid; MW = 296.4 g/mol) produced an absorbance of A412 nm = 1.025. If the extinction coefficient for the thiolate form of Ellman's reagent = 14,100 M-1-cm-1 (which arises when Ellman's reagent reacts with a single Cys-SH group), then how many free cysteine residues (Cys-SH) are in this protein? Sulfhydryl Molecule DTNB2- R-5 "O R-S -N* HS- Mixed Disulfide TNB2-
Transcribed Image Text:Treatment with Ellman's reagent (DTNB; (5,5')-difthig-bis-(2-nitrobenzoic acid; MW = 296.4 g/mol) produced an absorbance of A412 nm = 1.025. If the extinction coefficient for the thiolate form of Ellman's reagent = 14,100 M-1-cm-1 (which arises when Ellman's reagent reacts with a single Cys-SH group), then how many free cysteine residues (Cys-SH) are in this protein? Sulfhydryl Molecule DTNB2- R-5 "O R-S -N* HS- Mixed Disulfide TNB2-
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