Please answer this question and put them in order. O Macmillan LearningThreonine can be broken down by the enzyme threonine dehydratase, which catalyzes the conversion of threonine toa-ketobutyrate and ammonia as shown in part A of the figure. The enzyme uses PLP as a cofactor. The general mechanism ofPLP-dependent racemization is shown in part B of the figure.ABR- -C COO-HL-AminoacidEnz+ NH3Lys+H‚N+НО.H₂CCHHCH3-11-OHCH-CH-BRНО.NH3H₂C+NH-||COOCOOPLPThreoninedehydratase1~H+HOH₂C²Rс||+NHCHCH₂ CH₂COO1/mQuinonoid intermediatea-KetobutyrateHORH-C-COO-H₂CCOO + NH, + H,O+NH||CHLys-NH₂EnzRH-C+NH₂3COO-D-AminoacidPyridoxal phosphate(internal aldimine form,on regenerated enzyme)Arrange the steps in the reaction catalyzed by threonine dehydrates in order, using the racemization reaction shown in part B asa guide. Note that the threonine dehydrates reaction is not a racemization, but instead features an elimination at the ß carbonof threonine. Macmillan LearningArrange the steps in the reaction catalyzed by threonine dehydrates in order, using the racemization reaction shown in part B asa guide. Note that the threonine dehydrates reaction is not a racemization, but instead features an elimination at the ß carbonof threonine.First eventFinal eventAnswer BankThreonine forms an aldimine bond with PLP, displacing lysine.Reversal of the link to PLP releases dehydrated threonine from PLPProton abstraction at an a carbon leads to ß elimination of —OH.Hydrolysis of dehydrated threonine releases ammonia.Rearrangement of an enamine forms an imine.

Threonine dehydratase catalyzes the conversion of threonine to α-ketobutyrate , H2O and NH3 . The…

Answered: Arrange the steps in the reaction…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter26: Synthesis And Degradation Of Nucleotides

Section: Chapter Questions

Problem 16P

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Arrange the steps in the reaction catalyzed by threonine dehydrates in order, using the racemization reaction shown in part B as a guide. Note that the threonine dehydrates reaction is not a racemization, but instead features an elimination at the ß carbon of threonine.