Apparatus:●●●●●●●P1000 Gilson and blue gilson tips3 disposable cuvettesSpectrophotometerparafilmstop watchtissueMaterials and MethodsChemicals:These chemicals are toxic, gloves and eye protection must be worn. All spills must be reportedto the lecturer or a demonstrator and cleaned up immediately. Students must wash their handsbefore the laboratory is left.50 mM sodium phosphate buffer, pH 7.5 (20ml)Distilled water2 mM 2-nitrophenyl-ß-D-galactopyranoside (ONP-Gal) (5ml)2 mM 2-nitrophenyl-ß-glucopyranoside (ONP-Glu) (5ml)2 units.ml-¹ B-galactosidase (from E. coli) (10ml)Method:1. Check that the spectrophotometer is on and set at 440 nm. If it is not set up correctly ASK forhelp.3. In the second cuvette add:beslist elven2. Fill the first cuvette with 3 ml distilled water and zero the spectrophotometer by placing thecuvette in the machine in the correct orientation and pressing the BLUE button. If you follow theinstructions below you will not need to repeat this.1 ml 50 mM sodium phosphate buffer, pH 7.51 ml Distilled water0.5 ml 2-nitrophenyl-ß-D-galactopyranoside (ONP-Gal)add 0.5ml ß-galactosidase to the cuvette WHEN YOU ARE READY TO STARTTHE CLOCKMix by covering the top of the cuvette with parafilm, ensure the outside of the cuvette is dry andplace in the correct orientation (bevelled sides to the left and right) in the spectrophotometer.Press the GREEN button to start measuring the absorbance.4. Take your first absorbance reading at 30 seconds and continue taking readings every 30seconds for 5 minutes, record the reading in the form of a table (for table, see next page).5. REPEAT the experiment starting at step 3.6. Calculate the average from the 2 experiments for each time point. Plot your data on a graphand calculate the rate (absorbance units minute).Rate of the reaction based on your plotted values: Experiment 1Time (Minutes) Absorbance level (AU)0.120.1810.2740.3640.4510.5330.6060.6750.7340.7860.511.522.533.544.55

We have to plot the graph of absorbance vs time and determine the rate of the reaction.

Apparatus: Materials and Methods P1000 Gilson and blue gilson tips 3 disposable cuvettes Spectrophotometer parafilm stop watch tissue Chemicals: These chemicals are toxic, gloves and eye protection must be worn. All spills must be reported to the lecturer or a demonstrator and cleaned up immediately. Students must wash their hands before the laboratory is left. 50 mM sodium phosphate buffer, pH 7.5 (20ml) Distilled water 2 mM 2-nitrophenyl-ß-D-galactopyranoside (ONP-Gal) (5ml) 2 mM 2-nitrophenyl-ß-glucopyranoside (ONP-Glu) (5ml) 2 units.ml-¹ ß-galactosidase (from E. coli) (10ml) Method: 1. Check that the spectrophotometer is on and set at 440 nm. If it is not set up correctly ASK for help. 2. Fill the first cuvotto with f

Apparatus: Materials and Methods P1000 Gilson and blue gilson tips 3 disposable cuvettes Spectrophotometer parafilm stop watch tissue Chemicals: These chemicals are toxic, gloves and eye protection must be worn. All spills must be reported to the lecturer or a demonstrator and cleaned up immediately. Students must wash their hands before the laboratory is left. 50 mM sodium phosphate buffer, pH 7.5 (20ml) Distilled water 2 mM 2-nitrophenyl-ß-D-galactopyranoside (ONP-Gal) (5ml) 2 mM 2-nitrophenyl-ß-glucopyranoside (ONP-Glu) (5ml) 2 units.ml-¹ ß-galactosidase (from E. coli) (10ml) Method: 1. Check that the spectrophotometer is on and set at 440 nm. If it is not set up correctly ASK for help. 2. Fill the first cuvotto with f

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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