Analysis of the pathological constituentsGIVE THE FOLLOWING:- Name of Test/Test Reagent- Positive Result 1) Protein (Note: Presence of protein in urine is termed as proteinuria or albuminuria)A. Heat and Acetic acid testFill a test about ¾ full of urine.Heat the upper portion gently to boiling for 1 -2 minutes being careful not to shake the tube.Rotate the tube to prevent overheating. A turbidity may be due to albumin, phosphates or carbonates.Add 3 drops of acetic acid drop by drop while boiling between each drop. If turbidity disappears, it is due to carbonates and phosphates.B. Heller’s nitric acid testPlace 1 ml of urine sample in a test tube.Hold the test tube in an inclined position and carefully pour 1 drops of conc HNO3 down the side of the test tube.Note formation of white ring at the zone of contact of the two solutions which is an indication of the presence of protein.

Urinalysis can be defined as the series of tests conducted on the urine to check the condition of…

Answered: Analysis of the pathological…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Analysis of the pathological constituents

GIVE THE FOLLOWING:

- Name of Test/Test Reagent

- Positive Result

1) Protein (Note: Presence of protein in urine is termed as proteinuria or albuminuria)

A. Heat and Acetic acid test

Fill a test about ¾ full of urine.
Heat the upper portion gently to boiling for 1 -2 minutes being careful not to shake the tube.
Rotate the tube to prevent overheating. A turbidity may be due to albumin, phosphates or carbonates.
Add 3 drops of acetic acid drop by drop while boiling between each drop. If turbidity disappears, it is due to carbonates and phosphates.

B. Heller’s nitric acid test

Place 1 ml of urine sample in a test tube.
Hold the test tube in an inclined position and carefully pour 1 drops of conc HNO3 down the side of the test tube.
Note formation of white ring at the zone of contact of the two solutions which is an indication of the presence of protein.

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