amount of ibuprofen in the rat’s blood after one hour.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Next, a 10 mg/kg sample of ibuprofen was administered orally to a laboratory rat. A blood sample was drawn after one hour and gave a peak area of 80.2. Calculate the amount of ibuprofen in the rat’s blood after one hour.

Transcribed Image Text:An HPLC method was developed for the separation and determination of ibuprofen in rat plasma samples as part of a study of the time
course of the drug in laboratory animals. Several standards were chromatographed and the following results obtained:
Ibuprofen
Concentration,
Relative
Hg/mL
Peak Area
0.5
5.0
1.0
10.1
2.0
17.2
3.0
19.8
6.0
39.7
8.0
57.3
10.0
66.9
15.0
95.3
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