None Alexa Fluor 568 is a fluorescent dye that in aqueous solution displays amaximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 nsand a Stokes shift of 24 nm.(i) Show a schematic of a typical fluorimeter and its main components.Briefly explain the role of each component.(ii) Explain what is the "Stokes shift" and what physical processes underpinthe observation of such shifts; use diagrams and/or equations asappropriate in your explanation.(iii) Calculate the radiative decay constant of Alexa Fluor 568.

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Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter18: Raman Spectroscopy

Section: Chapter Questions

Problem 18.7QAP

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Alexa Fluor 568 is a fluorescent dye that in aqueous solution displays a maximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 ns and a Stokes shift of 24 nm. (i) Show a schematic of a typical fluorimeter and its main components. Briefly explain the role of each component. (ii) Explain what is the "Stokes shift" and what physical processes underpin the observation of such shifts; use diagrams and/or equations as appropriate in your explanation. (iii) Calculate the radiative decay constant of Alexa Fluor 568.
Express a wavelength of 442 nm as (a) a frequency. (b) a wavenumber.
Calculate the moment of inertia for HCl molecule from the given value of rotational constant, B = 10.40 cm. (A) 2.68 ×1047 kg m? (B) 4.21x10-1 kg m? (C) 2.68x10-51 kg m? 2 (D) 4.21 x10" kg m?
Inductively coupled plasma-optical emission spectroscopy (ICP-OES) is a quantitative method for determining trace elements in liquid samples. ICP-OES and other methods of spectrochemical analysis are typically subject to errors due to the present of interferences. It had been reported that ICP-OES is significantly affected by spectral interference, but relatively less affected by the chemical interferences. (i) Explain why ICP-OES is significantly affected by spectral interference but less affected by chemical interferences.
What is the Doppler-shifted wavelength of a red (680 nm) traffic light approached at 60 km h−1?
Give a clear handwritten answer...and explain
Q/ The bond length of the C0O molecule is 112.8 pm. Calculate the following (a) The reduced mass. (b) The rotational constant of CO when moment of inertia (I) is 1.4486 x 1046 kg.m² (c) Calculate the wavelength of the photon absorbed when a CO molecule initially in the J=2 level. makes a transition to the J=3 level.
The first three absorption lines in the pure rotational spec- trum of gaseous 12C16O are found to have the frequencies 1.15 x 1011, 2.30 × 1011, and 3.46 × 1011 s-1. Calculate: (a) The moment of inertia I of CO (in kg m²) (b) The energies of the J = 1, J = 2, and J = 3 rotational levels of CO, measured from the J = 0 state (in joules) (c) The C-O bond length (in angstroms)
4. Measurement momentum yield (A) +1 ħ. (C) 0 ħ. of the z component of angular for an electron in the 2px state will (B) (D) -1 ħ. ±1 h.
Q/ The bond length of the C0O molecule is 112.8 pm. Calculate the following (a) The reduced mass. (b) The rotational constant of CO when moment of inertia (I) is 1.4486 x 1046 kg.m² (c) Calculate the wavelength of the photon absorbed when a CO molecule initially in the J=2 level. makes a transition to the J=3 level.
Identify and provide either an concise explanation of the concept signal-to-noise ratio (SNR), in the context of chemical analysis.
Estimate the lifetime of a state that gives rise to a line of width (i) 200 MHz, (ii) 2.45 cm−1.

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