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Alexa Fluor 568 is a fluorescent dye that in aqueous solution displays a maximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 ns and a Stokes shift of 24 nm. (i) Show a schematic of a typical fluorimeter and its main components. Briefly explain the role of each component. (ii) Explain what is the "Stokes shift" and what physical processes underpin the observation of such shifts; use diagrams and/or equations as appropriate in your explanation. (iii) Calculate the radiative decay constant of Alexa Fluor 568.

Alexa Fluor 568 is a fluorescent dye that in aqueous solution displays a maximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 ns and a Stokes shift of 24 nm. (i) Show a schematic of a typical fluorimeter and its main components. Briefly explain the role of each component. (ii) Explain what is the "Stokes shift" and what physical processes underpin the observation of such shifts; use diagrams and/or equations as appropriate in your explanation. (iii) Calculate the radiative decay constant of Alexa Fluor 568.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Alexa Fluor 568 is a fluorescent dye that in aqueous solution displays a maximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 ns and a Stokes shift of 24 nm. (i) Show a schematic of a typical fluorimeter and its main components. Briefly explain the role of each component. (ii) Explain what is the "Stokes shift" and what physical processes underpin the observation of such shifts; use diagrams and/or equations as appropriate in your explanation. (iii) Calculate the radiative decay constant of Alexa Fluor 568.
Transcribed Image Text:Alexa Fluor 568 is a fluorescent dye that in aqueous solution displays a maximum absorbance at 576 nm, a quantum yield of 0.69, a lifetime of 3.60 ns and a Stokes shift of 24 nm. (i) Show a schematic of a typical fluorimeter and its main components. Briefly explain the role of each component. (ii) Explain what is the "Stokes shift" and what physical processes underpin the observation of such shifts; use diagrams and/or equations as appropriate in your explanation. (iii) Calculate the radiative decay constant of Alexa Fluor 568.
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