Acid phosphatases are an important group of enzymes that can be detected in human blood serum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze biological phosphate esters as follows; R-O-P-O3-2 + H 2O OR-OH + HO-P-O3-2. Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostate gland. The enzyme from the prostate gland is clinically important because an increased activity in the blood is frequently an indication of cancer of the prostate gland. Tartrate ion can strongly inhibit the phosphatase from the prostate gland, but not acid phosphatases from other tissues. How can you use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostate gland in human blood serum?
Acid phosphatases are an important group of enzymes that can be detected in human blood serum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze biological phosphate esters as follows; R-O-P-O3-2 + H 2O OR-OH + HO-P-O3-2. Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostate gland. The enzyme from the prostate gland is clinically important because an increased activity in the blood is frequently an indication of cancer of the prostate gland. Tartrate ion can strongly inhibit the phosphatase from the prostate gland, but not acid phosphatases from other tissues. How can you use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostate gland in human blood serum?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Transcribed Image Text:Acid phosphatases are an important group of enzymes that can be detected in human blood
serum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze biological
phosphate esters as follows;
R-O-P-O3-2 + H 2O OR-OH + HO-P-O3-2.
Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and
prostate gland. The enzyme from the prostate gland is clinically important because an increased
activity in the blood is frequently an indication of cancer of the prostate gland. Tartrate ion can
strongly inhibit the phosphatase from the prostate gland, but not acid phosphatases from other
tissues. How can you use the information above to develop a specific procedure for measuring the
activity of the acid phosphatase of the prostate gland in human blood serum?
Expert Solution
Step 1
Acid phosphatases are the enzymes that are responsible to hydrolyze organic phosphates when the pH (potential of hydrogen ions) is slightly acidic. They catalyze the reaction that involves the conversion of orthophosphoric monoester and a water molecule into phosphoric acid and alcohol.
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