Absorbance at Amax

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Calculate the transmittance (in %) of an unknown concentration of a protein that was standardized using the following data:
concentration of the reference
Absorbance at Amax
protein (mg / mL)
1.00
0.247
3.00
0.559
5.00
0.768
7.00
0.997
9.00
1.243
Absorbance of the unknown protein: 0.728
Provide your answer using 3 significant figures.
Transcribed Image Text:Calculate the transmittance (in %) of an unknown concentration of a protein that was standardized using the following data: concentration of the reference Absorbance at Amax protein (mg / mL) 1.00 0.247 3.00 0.559 5.00 0.768 7.00 0.997 9.00 1.243 Absorbance of the unknown protein: 0.728 Provide your answer using 3 significant figures.
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