A. Phosphate Buffer 1. Prepare 100 mL of a 0.01 M phosphate buffer, pH 7.70, from stock solutions of 0.1 M K2HPO4and 0.2 M KH2PO4. (pKa for the weak acid = 7.20). a. Use the Henderson-Hasselbalch equation to calculate the volume of each stock solution needed. pH = RKa + log [conjugate base] / [weak acid] b. Check your calculations with other students. See the instructor if there is uncertainty. c. Make the solution and check the pH of a portion of your buffer solution using the pH meter.
A. Phosphate Buffer 1. Prepare 100 mL of a 0.01 M phosphate buffer, pH 7.70, from stock solutions of 0.1 M K2HPO4and 0.2 M KH2PO4. (pKa for the weak acid = 7.20). a. Use the Henderson-Hasselbalch equation to calculate the volume of each stock solution needed. pH = RKa + log [conjugate base] / [weak acid] b. Check your calculations with other students. See the instructor if there is uncertainty. c. Make the solution and check the pH of a portion of your buffer solution using the pH meter.
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![A. Phosphate Buffer
1. Prepare 100 mL of a 0.01 M phosphate buffer, pH 7.70, from stock solutions of
0.1 M K2HPO4and 0.2 M KH2PO4. (pKa for the weak acid = 7.20).
a. Use the Henderson-Hasselbalch equation to calculate the volume of each stock
solution needed.
pH = RKa + log [conjugate base] / [weak acid]
b. Check your calculations with other students. See the instructor if there is
uncertainty.
c. Make the solution and check the pH of a portion of your buffer solution using the
pH meter.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F683fd89e-6b36-42e1-86cf-6d61a4d4eaa4%2Fabdbfd89-ec3e-4708-ba03-ae8629bc1f7d%2F30x6s2n_processed.png&w=3840&q=75)
Transcribed Image Text:A. Phosphate Buffer
1. Prepare 100 mL of a 0.01 M phosphate buffer, pH 7.70, from stock solutions of
0.1 M K2HPO4and 0.2 M KH2PO4. (pKa for the weak acid = 7.20).
a. Use the Henderson-Hasselbalch equation to calculate the volume of each stock
solution needed.
pH = RKa + log [conjugate base] / [weak acid]
b. Check your calculations with other students. See the instructor if there is
uncertainty.
c. Make the solution and check the pH of a portion of your buffer solution using the
pH meter.
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