A. Identify the independent variable in this experiment. ____   B. Describe the role of both the light AND the DCPIP in this experiment. ____   C. Explain the results comparing samples 1 and 6 and samples 2 and 3.  _____ D. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6.  _____

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A. Identify the independent variable in this experiment. ____

 

B. Describe the role of both the light AND the DCPIP in this experiment. ____

 

C. Explain the results comparing samples 1 and 6 and samples 2 and 3.  _____


D. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6.  ______

Question 1:
Photosynthetic activity can be measured using chloroplasts suspended in a buffered solution containing DCPIP, a
dye that can accept electrons from the electron transport chain of photosynthesis. Transfer of electrons to DCPIP
decreases the relative absorbance of a specific wavelength of light (605 nm) by a solution that contains the dye.
A buffered solution containing chloroplasts and DCPIP was divided equally among six identical samples. The
samples were placed at various distances from a lamp, and then all samples were exposed to white light from the
lamp for 60 minutes at room temperature. Sample 3 was wrapped in foil to prevent any light from reaching the
solution. At 20-minute intervals, the photosynthetic activity in each sample was determined by measuring the
relative absorbance of 605 nm light. See the results of the experiment below
Transcribed Image Text:Question 1: Photosynthetic activity can be measured using chloroplasts suspended in a buffered solution containing DCPIP, a dye that can accept electrons from the electron transport chain of photosynthesis. Transfer of electrons to DCPIP decreases the relative absorbance of a specific wavelength of light (605 nm) by a solution that contains the dye. A buffered solution containing chloroplasts and DCPIP was divided equally among six identical samples. The samples were placed at various distances from a lamp, and then all samples were exposed to white light from the lamp for 60 minutes at room temperature. Sample 3 was wrapped in foil to prevent any light from reaching the solution. At 20-minute intervals, the photosynthetic activity in each sample was determined by measuring the relative absorbance of 605 nm light. See the results of the experiment below
Relative Absorbance of 605 nm Light (arbitrary units)
Sample
Distance
O min
20 min
40 min
60 min|
from lamp
(cm)
1
15
0.89
0.61
0.34
0.04
2
30
0.90
0.67
0.41
0.14
3*
30
0.88
0.87
0.86
0.87
4
45
0.86
0.69
0.47
0.26
5
60
0.92
0.75
0.59
0.41
75
0.88
0.79
0.71
0.58
*Wrapped in foil
Transcribed Image Text:Relative Absorbance of 605 nm Light (arbitrary units) Sample Distance O min 20 min 40 min 60 min| from lamp (cm) 1 15 0.89 0.61 0.34 0.04 2 30 0.90 0.67 0.41 0.14 3* 30 0.88 0.87 0.86 0.87 4 45 0.86 0.69 0.47 0.26 5 60 0.92 0.75 0.59 0.41 75 0.88 0.79 0.71 0.58 *Wrapped in foil
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 Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which one is to be answered, we are answering only the first three questions for you. If you need help with the other questions too, please repost them separately.

    Plant cells are capable of photosynthesis because of the presence of the light- absorbing green chlorophyll pigment in the chloroplasts. Photosynthesis is a physiochemical process by which photosynthetic organisms convert light energy to chemical energy in the form of reducing power as NADPH and ATP and use these chemicals to carry out carbon dioxide fixation. There are two-stages in the photosynthesis process: one is light dependent, and one is light-independent

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