A. Describe the role of both the light AND the DCPIP in this experiment. B. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6
A. Describe the role of both the light AND the DCPIP in this experiment. B. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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A. Describe the role of both the light AND the DCPIP in this experiment.
B. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6
![A buffered solution containing chloroplasts and DCPIP was divided equally among six identical samples. The
samples were placed at various distances from a lamp, and then all samples were exposed to white light from the
lamp for 60 minutes at room temperature. Sample 3 was wrapped in foil to prevent any light from reaching the
solution. At 20-minute intervals, the photosynthetic activity in each sample was determined by measuring the
relative absorbance of 605 nm light. See the results of the experiment below
Relative Absorbance of 605 nm Light (arbitrary units)
Sample
0 min
60 min
Distance
from lamp
(cm)
20 min
40 min
1
15
0.89
0.61
0.34
0.04
2
30
0.90
0.67
0.41
0.14
3*
30
0.88
0.87
0.86
0.87
4
45
0.86
0.69
0.47
0.26
5
60
0.92
0.75
0.59
0.41
6
75
0.88
0.79
0.71
0.58
*Wrapped in foil
LO](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff8cbb2f3-e5dd-44a1-b0ef-121ff2ef58fa%2F4fb6ee4f-f2e9-4c82-b218-f172ad44530f%2F8onz0h_processed.png&w=3840&q=75)
Transcribed Image Text:A buffered solution containing chloroplasts and DCPIP was divided equally among six identical samples. The
samples were placed at various distances from a lamp, and then all samples were exposed to white light from the
lamp for 60 minutes at room temperature. Sample 3 was wrapped in foil to prevent any light from reaching the
solution. At 20-minute intervals, the photosynthetic activity in each sample was determined by measuring the
relative absorbance of 605 nm light. See the results of the experiment below
Relative Absorbance of 605 nm Light (arbitrary units)
Sample
0 min
60 min
Distance
from lamp
(cm)
20 min
40 min
1
15
0.89
0.61
0.34
0.04
2
30
0.90
0.67
0.41
0.14
3*
30
0.88
0.87
0.86
0.87
4
45
0.86
0.69
0.47
0.26
5
60
0.92
0.75
0.59
0.41
6
75
0.88
0.79
0.71
0.58
*Wrapped in foil
LO
Expert Solution
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Step 1
Light is one of the main raw materials in the process of photosynthesis. All photosynthetic organisms contain photosynthetic pigments that can absorb light such as chlorophyll "a" and it absorbs light in violet-blue and reddish orange-red wavelengths. The amount of light absorbed by the pigments determines the rate of photosynthesis plants.
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