1. This question is adapted from Harris 19-D.Carnosine is a dipeptide whose antioxidant properties protect cells from free radicals. Carnosine wasdetermined by derivatization with naphthalene-2,3-dicarboxyaldehyde and cyanide followed byfluorescence detection using excitation at 445 nm and emission at 490. Quantification was by standardaddition. To four aliquots of 20-ul cell lysate were added volumes of 100µM carnosine standard togenerate final concentrations of 0, 1.0, 2.5, and 5.0 µM added carnosine. Solutions were diluted to 70µlbefore addition of 15µl of 5 mM naphthalene-2,3-dicarboxyaldehyde and 15µl of 10 mM NaCN.HH2N.OHCarnosineHN.Naphthalene-2,3-dicarboxyaldehydeCyanideOHFluorescent productHN.Fluorescence intensityConcentration (uM) of added carnosinein final 100 µL0.00.4651.00.6982.51.0295.01.651 a) Write out a step by step procedure for this question, if you were to perform it in the labyourself. Include all steps. If drawing a sketch helps you keep track, you may do so.b) What glassware and lab equipment would you need?c) What is the concentration of carnosine in your cell lysate? If you use excel at any point,please include a copy of your workings.

Prepare Carnosine Standards: a. Pipette 20 µL of cell lysate into four separate vials. b. Add 100 µM…

a) Write out a step by step procedure for this question, if you were to perform it in the lab yourself. Include all steps. If drawing a sketch helps you keep track, you may do so. b) What glassware and lab equipment would you need? c) What is the concentration of carnosine in your cell lysate? If you use excel at any point, please include a copy of your workings.

a) Write out a step by step procedure for this question, if you were to perform it in the lab yourself. Include all steps. If drawing a sketch helps you keep track, you may do so. b) What glassware and lab equipment would you need? c) What is the concentration of carnosine in your cell lysate? If you use excel at any point, please include a copy of your workings.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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