3. Take the 1M solution, and make 100µl of a 250µM solution. You cannot pipette less than 1µL, so you may need to do this in more than one step. Please show all calculations. 4. You have a 20OµM solution of a primer (40nt) in a volume of 300µL, how many µg of primer do you have total? 5. You received a primer (25nt) which came to you as a powder with a mass of 0.21mg. 1. What concentration of primer do you have when you add 100µL water? 2. How would you make a 5pmol/uL solution with a total volume of 100µL?

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Introduction to chemistry

Chemistry is a branch of science that deals with the analysis of material and the substances that comprise it. It also manages the properties of these substances and their reactions to form new substances. It is basically centered on particles and atoms that make up components and mixtures. These chemical species will in general connect using bonds.