a) What was the full peptide sequence before degradation? b) Paula thought she forgot to treat her peptide sample with iodoacetate but was relieved to see the results. What could have gone through her mind?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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4
Using Edman's degradation and mass spectrometry, a polypeptide is cut into the following
fragments:
Chymotrypsin
Trypsin
ASHREKGQATARGY
MATSLAR
ARMTKNVW
NVWASHR
MКАСE
GQATAR
MATSL
GYMK
MTK
ACE
EK
a) What was the full peptide sequence before degradation?
b) Paula thought she forgot to treat her peptide sample with iodoacetate but was relieved to
see the results. What could have gone through her mind?
Transcribed Image Text:Using Edman's degradation and mass spectrometry, a polypeptide is cut into the following fragments: Chymotrypsin Trypsin ASHREKGQATARGY MATSLAR ARMTKNVW NVWASHR MКАСE GQATAR MATSL GYMK MTK ACE EK a) What was the full peptide sequence before degradation? b) Paula thought she forgot to treat her peptide sample with iodoacetate but was relieved to see the results. What could have gone through her mind?
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