A researcher is sequencing a peptide using Edman degradation. However upon examination of the PTH-amino acid products, using HPLC, one of the amino acids does not correspond to any of the amino acid standards. Why? O This amino acid is a non-standard amino acid O The PTC-peptide cleaved at the second amino acid residue O Two PTH molecules reacted with the N-terminal amino group O The pH of the buffer solution is too low in the HPLC column

Q: A mixture of Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74), Phenylalanine (pl 5.48)…

A: In chromatography, we've got a go with the flow coming out of a column, when we inject a substance…

Q: In a sample solution given to be analyzed; Starch + Val-Gly-Ile-Trp-Lys-Gly-Ala-Gly-Val-Glu  Plan…

A: Peptide bond binds the amino acid together and forms a linear chain ,more than 10 amino acids are…

Q: Which one of the following would best allow us to conclude that the rate of chymotrypsin was…

A: Chymotrypsin is a serine protease enzyme that aids in digestion in several organisms including…

Q: You have performed protein purification on your new favorite enzyme using a protocol which involves…

A: Proteins are formed of amino acid chains. They differ in molecular size, electric charge,…

Q: Determine the sequence of the decapeptide given the following information below. The decapeptide was…

A: Decapeptide is a peptide that contains 10 amino acids in it. The amino acids present are 3 units of…

Q: VII. Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the…

A: Differential hydrolysis involve fragmentation in the peptide bond backbone using different enzymes.…

Q: The sequence of a 29 aa long peptide can be determined from the following data: Treatment of the…

A: A peptide is an amino acid chain that is small in length. The amino acids in a peptide are linked…

Q: hymotrypsin digestion, separation of peptides, and Edmann technique give the sequences for peptide…

A: Peptidases are enzymes that act on the peptide bond. Endopeptidases are enzymes that catalyze the…

Q: Which of the following peptides are consistent with the data below? 1. Amino acid analysis gave Met,…

A:

Q: a protein pretreated with an sds page detergent shows two bands around 100 and 150 kD on the…

A: SDS imparts an even charge and linearises your macromolecule. Beta-mercaptoethanol breaks down the…

Q: High salt concentrations tend to cause protein aggregation. Suggest a way to identify proteins…

A: Salting out is a process based on protein solubility. The globular proteins are precipitate in the…

Q: Please provide a separation solution of combining two types of chromatography to isolate the protein…

A: Here, we can see that two proteins (protein A and protein C) are non-polar in nature while the other…

Q: calculate the molecular weights of proteins

A: Amino acids are the monomer units of peptides and proteins and they vary from 89 Dalton (Alanine) to…

Q: An oligopeptide ALVGALGATPTPQMWSHSWRGVSIKS was digested with trypsin.Which method would be most…

A: CHROMATOGRAPHY It is a technique of separation of molecules on the basis of solubility into two…

Q: Examine the following peptide and the inserted table, and answer the 2 questions below:…

A: The net charge of peptide molecule depends upon the charge on the amino acids side chain, N-terminal…

Q: Wh are doing this procedures can you explain? (ex. heating or adding chemicals etc.) 1)…

A: There are various analytical and preparative methods in protein purification. The commonly used four…

Q: Why different amino acids have different Rf values? If you separate a mixture of amino acids consist…

A: Paper chromatography is the separation technique where the mixture of the amino acids was separated…

Q: A decapeptide was analyzed and the following information obtained. Determine the primary structure…

A: Proteins are polymers of amino acids. Amino acids are bound together by peptide bonds. An amino acid…

Q: H CH₂ HỎ=CH. CH₂ H₂C (5) H CH₂ H₂C CH₂ CH₂ CH₂ ΝΗ C=NH NH₂ a) Which of the following statements…

A: Recall that:amino acid sequences are written with N-terminal amino acid on the left and C-terminal…

Q: One of the reasons for oxidizing the disulfide bonds in RNase A before removing the urea in the…

A: In the Anfinsen experiment, Christian Anfinsen aimed to investigate the relationship between the…

Q: Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the following…

A: The average molecular weight of 1 amino acid is 110 Da. It is given that the molecular weight of the…

Q: Discuss the reaction conditions you would need to carry out the PEGylation reaction of this peptide…

A: In order to solve a variety of biopharmaceutical inadequacies, the word "PEGylation" refers to the…

Q: Amino acid analysis of the peptide gave the following residues: Asp Leu Lys Met Phe Tyr. The…

A: Edman degradation of a peptide results in the release of the N-terminal amino acid of the peptide as…

Q: A protein was purified and then analyzed on a size exclusion column. Its MW was determined to be…

A: SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is used for separating proteins…

Q: A mixture of Asparagine (pl 5.41) , Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74)…

A: Cation exchange chromatography separates the molecules based on their net negative charge. Cation…

Q: A tetrapeptide, glutamate-glycine-alanine-lysine, is prepared at at concentration of 1 mM (0.001 M)…

A: The Beer-Lambert law is a relationship between the atteunation of light through a substance and the…

Q: The octapeptide gly-cys-met-asn-lys-ala-tyr-gly was hydrolyzed consecutively by CNBr and then…

A: The octapeptide gly-cys-met-asn-lys-ala-tyr-gly was hydrolyzed consecutively by CNBr and then…

Q: At which pH would the peptide with the amino acid sequence 'KEYHR' be found in an IPG (Immobilized…

A: Recall that:A peptide is a short chain of amino acid residues linked together via a peptide bond.…

Q: A peptide with endorphin-like property was produced and obtained from the blood. This was sequence…

A: Protein degradation is the process of protein digestion using proteases. Proteases specifically…

Q: Which of the peptide sequences below best matches the hydropathy plot shown? L 10 Residue Number 4 2…

A: Hydrophobicity plot analyzes the amount of the hydrophobicity and the hydrophilicity that is present…

Q: You are analyzing the peptide ala-ile-glu-lys-phe-val- tyr-cys. If you treat the peptide with…

A: Proteins or peptides are a linear chain of amino acids attached together via peptide bonds. Natural…

Q: Determine the pI of the peptide H2N-Ala-Lys-Ser-Arg-COOH at pH 11, please explain why some pKas are…

A: Amino acids are the monomeric unit of protein. There are 20 amino acids having basic common…

Q: A researcher has synthesized two peptides and needs to confirm whether the peptides were synthesized…

A: Tandem MS(also called MS/MS) and ElectroSpray Ionization-Time Of Flight Mass Spectroscopy…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.