A pure culture of Nitrosomonas was grown at 20°C under batch conditions. The following experimental data were collected. Time (h) 2.0 5.0 10.0 20.0 25.0 30.0 Nitrosomonas Concentration, X, (mg/L) 102 105 117 123 130 137 Rearranging of Equation (4.50) results in the following equation which can be used for determining the specific growth rate of the culture. In(X₂) = ln(X) + µt A plot of ln(X₂) versus time (t) will yield a straight line with the slope equal to μ and the y-intercept equal to In(X) or Xo = ex-Intercept. Determine the specific growth rate μ.

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
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### Nitrosomonas Culture Growth at 20°C

A pure culture of *Nitrosomonas* was grown at 20°C under batch conditions. The following experimental data were collected:

| Time (h) | *Nitrosomonas* Concentration, \( X_t \) (mg/L) |
|----------|--------------------------------------------|
| 2.0      | 102                                        |
| 5.0      | 105                                        |
| 10.0     | 117                                        |
| 20.0     | 123                                        |
| 25.0     | 130                                        |
| 30.0     | 137                                        |

#### Determining the Specific Growth Rate

By rearranging Equation (4.50), the following equation can be used to determine the specific growth rate of the culture:

\[
\ln(X_t) = \ln(X_0) + \mu t
\]

A plot of \(\ln(X_t)\) versus time (\(t\)) will yield a straight line with:
- The slope equal to \(\mu\)
- The y-intercept equal to \(\ln(X_0)\) or \(X_0 = e^{\text{Y-Intercept}}\)

Determine the specific growth rate \(\mu\) using this linear relationship.
Transcribed Image Text:### Nitrosomonas Culture Growth at 20°C A pure culture of *Nitrosomonas* was grown at 20°C under batch conditions. The following experimental data were collected: | Time (h) | *Nitrosomonas* Concentration, \( X_t \) (mg/L) | |----------|--------------------------------------------| | 2.0 | 102 | | 5.0 | 105 | | 10.0 | 117 | | 20.0 | 123 | | 25.0 | 130 | | 30.0 | 137 | #### Determining the Specific Growth Rate By rearranging Equation (4.50), the following equation can be used to determine the specific growth rate of the culture: \[ \ln(X_t) = \ln(X_0) + \mu t \] A plot of \(\ln(X_t)\) versus time (\(t\)) will yield a straight line with: - The slope equal to \(\mu\) - The y-intercept equal to \(\ln(X_0)\) or \(X_0 = e^{\text{Y-Intercept}}\) Determine the specific growth rate \(\mu\) using this linear relationship.
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A pure culture of Nitrosomonas was grown at 20 degree C under batch conditions. The following experimental data were collected :

TIME(h)Nitrosomonas Concentration, Xt (mg/L)
2102
5105
10117
20123
25130
30137
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