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A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: |Replicate Amount of product |Concentration of total protein in worm extract | (µg/ml) Locality liberated over 10 minutes (pmol) 101 87 107 Hokkaido 1 4 3 3 4 4 88 3 89 5 126 120 Hon-shu 1 2 3 3 156 4 4 153 5 5 119 4 Kyu-shu 1 103 4 99 3 94 6 4 108 3 110 Okinawa 1 88 3 90 4 3 96 4 100 5 5 112 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean ± standard error (n=5).

A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: |Replicate Amount of product |Concentration of total protein in worm extract | (µg/ml) Locality liberated over 10 minutes (pmol) 101 87 107 Hokkaido 1 4 3 3 4 4 88 3 89 5 126 120 Hon-shu 1 2 3 3 156 4 4 153 5 5 119 4 Kyu-shu 1 103 4 99 3 94 6 4 108 3 110 Okinawa 1 88 3 90 4 3 96 4 100 5 5 112 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean ± standard error (n=5).

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Locality Replicate Amount of product Concentration of total protein in worm extract (µg/ml) liberated over 10 minutes (pmol) Hokkaido 1 101 4 87 3 3 107 4 4 88 3 5 89 Hon-shu 1 126 2 2 120 3 3 156 4 4 153 5 5 119 4 Куu-shu 1 103 4 2 99 5 3 94 4 108 3 5 110 5 Okinawa 1 88 3 2 90 4 3 96 4 100 112 5 5 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean + standard error (n=5).
Transcribed Image Text:A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Locality Replicate Amount of product Concentration of total protein in worm extract (µg/ml) liberated over 10 minutes (pmol) Hokkaido 1 101 4 87 3 3 107 4 4 88 3 5 89 Hon-shu 1 126 2 2 120 3 3 156 4 4 153 5 5 119 4 Куu-shu 1 103 4 2 99 5 3 94 4 108 3 5 110 5 Okinawa 1 88 3 2 90 4 3 96 4 100 112 5 5 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean + standard error (n=5).
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