(a) In High Performance Liquid Chromatography (HPLC), the separation method is by partition of the solute (analyte) between the mobile phase and the stationary phase. Explain how this leads to separation of analyte mixtures; (b) Describe the structure and materials use in a typical column used for HPLC. Thereby explain why high pressures have to be used in HPLC; (c) Discuss how the need for high pressures also imposes requirements on the injector;
(a) In High Performance Liquid Chromatography (HPLC), the separation method is by partition of the solute (analyte) between the mobile phase and the stationary phase. Explain how this leads to separation of analyte mixtures; (b) Describe the structure and materials use in a typical column used for HPLC. Thereby explain why high pressures have to be used in HPLC; (c) Discuss how the need for high pressures also imposes requirements on the injector;
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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