A group of students were able to isolate a novel amylase from the saliva of a pig found in Country Aeros. In the process of isolating the novel saliva amylase, the students wanted to prepare 250 mL of 1.0 M phosphate buffer with a pH = 7.4. (AW (g/mol): Na = 23.10, H = 1.01, P = 30.97, O=16.00) Base from this table, what buffer system - acid, conjugate base- is most suitable to prepare the buffer? Acid Ка H;PO4 HPO. НРО2 1x10з 6.3 x10-8 4.2х 1013

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A group of students were able to isolate a novel amylase from the saliva of a pig found in
Country Aeros. In the process of isolating the novel saliva amylase, the students wanted to
prepare 250 mL of 1.0 M phosphate buffer with a pH = 7.4. (AW (g/mol): Na = 23.10, H = 1.01,
P = 30.97, O=16.00)
Base from this table, what buffer system - acid, conjugate base- is most suitable to prepare the
buffer?
Acid
Ка
H3PO4
1x10з
6.3 х108
HPO,?
4.2 x 10-13
Transcribed Image Text:A group of students were able to isolate a novel amylase from the saliva of a pig found in Country Aeros. In the process of isolating the novel saliva amylase, the students wanted to prepare 250 mL of 1.0 M phosphate buffer with a pH = 7.4. (AW (g/mol): Na = 23.10, H = 1.01, P = 30.97, O=16.00) Base from this table, what buffer system - acid, conjugate base- is most suitable to prepare the buffer? Acid Ка H3PO4 1x10з 6.3 х108 HPO,? 4.2 x 10-13
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