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a) Do the loss of function phenotypes differ between the genes between the genes in figure 1? What does this mean? b) What are the two cell types responsible for producing maternal gene products involved in terminal patterning in the Drosophila cocyte? In which of these two cell types is each of the three genes expressed? c) Based on the phenotypes you observed for the three genes, and on the differences in their expression patterns, which one of the genes do you think is the localised determinant of terminal patterning? d❘ From the gain-of-function experiments, do trunk and torso like work upstream or downstream of torso? Explain. (4 m e) How might you test which of the trunk and torso-like genes acts upstream of the other? f) The torso gene has been shown to have another important role in Drosophila development outside of terminal patterning. What do you think would be a good first experiment to do to begin to investigate possible other roles for the trunk or torso - like genes? C a b d e CD g f h Figure 2: (a) represents the wild type, (b) represents torso gene loss of function, (c) represents trunk gene loss of function, (d) represents torso-like gene loss of function, (e) represents torso gene loss of function. (f) represents torso gene gain of function, (g) represents torso gene gain of function and trunk gene loss of function, (h) represents torso gene gain of function and torso-like gene loss of function Control Loss-of-function (LOF) Gain-of-function (GOF) Mis-expression wildtype torso% torso GOF c355-GAL4 UAS-torso-like trunk/ torso GOF trunk/ torso-like- torso GOF torso-like-

a) Do the loss of function phenotypes differ between the genes between the genes in figure 1? What does this mean? b) What are the two cell types responsible for producing maternal gene products involved in terminal patterning in the Drosophila cocyte? In which of these two cell types is each of the three genes expressed? c) Based on the phenotypes you observed for the three genes, and on the differences in their expression patterns, which one of the genes do you think is the localised determinant of terminal patterning? d❘ From the gain-of-function experiments, do trunk and torso like work upstream or downstream of torso? Explain. (4 m e) How might you test which of the trunk and torso-like genes acts upstream of the other? f) The torso gene has been shown to have another important role in Drosophila development outside of terminal patterning. What do you think would be a good first experiment to do to begin to investigate possible other roles for the trunk or torso - like genes? C a b d e CD g f h Figure 2: (a) represents the wild type, (b) represents torso gene loss of function, (c) represents trunk gene loss of function, (d) represents torso-like gene loss of function, (e) represents torso gene loss of function. (f) represents torso gene gain of function, (g) represents torso gene gain of function and trunk gene loss of function, (h) represents torso gene gain of function and torso-like gene loss of function Control Loss-of-function (LOF) Gain-of-function (GOF) Mis-expression wildtype torso% torso GOF c355-GAL4 UAS-torso-like trunk/ torso GOF trunk/ torso-like- torso GOF torso-like-

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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a) Do the loss of function phenotypes differ between the genes between the genes in figure 1? What does this mean? b) What are the two cell types responsible for producing maternal gene products involved in terminal patterning in the Drosophila cocyte? In which of these two cell types is each of the three genes expressed? c) Based on the phenotypes you observed for the three genes, and on the differences in their expression patterns, which one of the genes do you think is the localised determinant of terminal patterning? d❘ From the gain-of-function experiments, do trunk and torso like work upstream or downstream of torso? Explain. (4 m e) How might you test which of the trunk and torso-like genes acts upstream of the other? f) The torso gene has been shown to have another important role in Drosophila development outside of terminal patterning. What do you think would be a good first experiment to do to begin to investigate possible other roles for the trunk or torso - like genes? C a b d e CD g f h Figure 2: (a) represents the wild type, (b) represents torso gene loss of function, (c) represents trunk gene loss of function, (d) represents torso-like gene loss of function, (e) represents torso gene loss of function. (f) represents torso gene gain of function, (g) represents torso gene gain of function and trunk gene loss of function, (h) represents torso gene gain of function and torso-like gene loss of function Control Loss-of-function (LOF) Gain-of-function (GOF) Mis-expression wildtype torso% torso GOF c355-GAL4 UAS-torso-like trunk/ torso GOF trunk/ torso-like- torso GOF torso-like-
Transcribed Image Text:a) Do the loss of function phenotypes differ between the genes between the genes in figure 1? What does this mean? b) What are the two cell types responsible for producing maternal gene products involved in terminal patterning in the Drosophila cocyte? In which of these two cell types is each of the three genes expressed? c) Based on the phenotypes you observed for the three genes, and on the differences in their expression patterns, which one of the genes do you think is the localised determinant of terminal patterning? d❘ From the gain-of-function experiments, do trunk and torso like work upstream or downstream of torso? Explain. (4 m e) How might you test which of the trunk and torso-like genes acts upstream of the other? f) The torso gene has been shown to have another important role in Drosophila development outside of terminal patterning. What do you think would be a good first experiment to do to begin to investigate possible other roles for the trunk or torso - like genes? C a b d e CD g f h Figure 2: (a) represents the wild type, (b) represents torso gene loss of function, (c) represents trunk gene loss of function, (d) represents torso-like gene loss of function, (e) represents torso gene loss of function. (f) represents torso gene gain of function, (g) represents torso gene gain of function and trunk gene loss of function, (h) represents torso gene gain of function and torso-like gene loss of function Control Loss-of-function (LOF) Gain-of-function (GOF) Mis-expression wildtype torso% torso GOF c355-GAL4 UAS-torso-like trunk/ torso GOF trunk/ torso-like- torso GOF torso-like-
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