a) Circle or highlight with yellow coloring each essential amino acid residue that appears in the hirudin sequence. b) The prototropic groups and their stoichiometry in hirudin are listed below. Calculate the charge on hirudin at pH values 3 and 4 and then estimate its isoionic point.
a) Circle or highlight with yellow coloring each essential amino acid residue that appears in the hirudin sequence. b) The prototropic groups and their stoichiometry in hirudin are listed below. Calculate the charge on hirudin at pH values 3 and 4 and then estimate its isoionic point.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
a) Circle or highlight with yellow coloring each essential amino acid residue that appears in the hirudin sequence.
b) The prototropic groups and their stoichiometry in hirudin are listed below. Calculate the charge on hirudin at pH values 3 and 4 and then estimate its isoionic point.
Transcribed Image Text:2. Hirudin is a 65-amino acid polypeptide secreted from the salivary glands of leeches that binds to
thrombin and functions as an anti-coagulant in the blood stream. Its amino acid sequence is given
below according to 3-letter abbreviations. In its native state, all of the cysteine residues form oxidized,
disulfide bridges.
Leu-Thr-Tyr-Thr-Asp-Cys-Thr-Glu-Ser-Gly-Gln-Asn-Leu-Cys-Leu-Cys-Glu-Gly-
Ser-Asn-Val-Cys-Gly-Gln-Gly-Asn-Lys-Cys-Ile-Leu-Gly-Ser-Asp-Gly-Glu-Lys-
Asn-Gln-Cys-Val-Thr-Gly-Glu-Gly-Thr-Pro-Lys-Pro-Gln-Ser-His-Asn-Asp-Gly-
Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-Gln
Transcribed Image Text:Number/
Hirudin molecule
4
8
1
3
1
1
Amino Acid
Residue
Aspartate
Glutamate
Histidine
Lysine
Tyrosine
terminal -NH3Ⓡ
terminal -COOH
pKa
4.0
4.0
6.0
10.5
10.0
7.0
4.0
ΣΖ
Charge con-
tribution at
pH 3
The overall charge of thrombin is given in the table to the right as a
function of pH. What is the minimum pH above which hirudin can
bind to thrombin, /.e., estimate the isoionic point?
Charge con-
tribution at
pH 4
Charge on
Thrombin molecule
+39.2
+16.6
+10.2
-0.2
pH
3.5
5.5
7.5
9.5
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