A chromatographer fed up with waiting 36 minutes to accomplish a separation requiring 8000 plates, packs a new column to reduce separation time. She slashed the particle diameter by a factor of 3. She adjusted the flow rate to work at the same reduced velocity as before ( the h versus v curves for the two columns are the same) and chooses a column length to get the same number of plates. Comparing the new column to the old, by what factor will the following parameters change: a) plate height b) column length c) flow velocity d) pressure drop e) separation time? What is the new separation time?

Elements Of Electromagnetics
7th Edition
ISBN:9780190698614
Author:Sadiku, Matthew N. O.
Publisher:Sadiku, Matthew N. O.
ChapterMA: Math Assessment
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A chromatographer fed up with waiting 36
minutes to accomplish a separation requiring
8000 plates, packs a new column to reduce
separation time. She slashed the particle
diameter by a factor of 3. She adjusted the
flow rate to work at the same reduced
velocity as before ( the h versus v curves for
the two columns are the same) and chooses a
column length to get the same number of
plates.
Comparing the new column to the old, by
what factor will the following parameters
change:
a) plate height
b) column length
c) flow velocity
d) pressure drop
e) separation time?
What is the new separation time?
Transcribed Image Text:A chromatographer fed up with waiting 36 minutes to accomplish a separation requiring 8000 plates, packs a new column to reduce separation time. She slashed the particle diameter by a factor of 3. She adjusted the flow rate to work at the same reduced velocity as before ( the h versus v curves for the two columns are the same) and chooses a column length to get the same number of plates. Comparing the new column to the old, by what factor will the following parameters change: a) plate height b) column length c) flow velocity d) pressure drop e) separation time? What is the new separation time?
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