A biochemist wants to separate two peptides by ion-exchange chromatography. At the pH of the mobile phase to be used on the column,one peptide (A) has a net charge of −3 due to the presence of more Glu andAsp residues than Arg, Lys, and His residues. Peptide B has a net charge of+1. Which peptide would elute first from a cation-exchange resin? Whichwould elute first from an anion-exchange resin?

Q: For the amino acid tryptophan: Draw its complete protonic equilibria. Indicate the net charge of…

A: The proteins are composed of twenty naturally occurring amino acids. The net charge on an amino acid…

Q: List all the amino acids you would expect to bind to a cation-exchange column at pH 2.

A: Chromatography is derived from two Greek words, chroma means colour and graphein means to write.…

Q: Under certain conditions, peptide bond formation rather than peptide bond hydrolysis is…

A: Peptide bond formation- A peptide bond is a chemical bond formed between two molecules when the…

Q: Chymotrypsin is a pH dependent protein. Enzymatic activity is the greatest between pH=7.0 to pH=8.5.…

A: Chymotrypsin is a protease that is secreted by the pancreas. It cleaves the peptide bond next to the…

Q: if the reaction pH drops significantly (pH=2 for example), how would reaction catalyzed be serine…

A: Serine protease are the enzymes that cleave peptide bonds in proteins in which serine serves as…

Q: What are hydrophobic interactions? What are two ways that hydrophobic interactions might impact…

A: Introduction: Large macromolecules known as proteins are composed of lengthy chains of amino acids.…

Q: The figure shows binding curves for two proteins that bind the same ligand. Which binding curve…

A: Ligand binding curve: this is also called as saturation curve that shows the fraction Y of protein…

Q: In a solution with pH ~7 most of the amino acids are form zwitterions. Name the amino acids which…

A: Amino acids: a. Amino acids are the building block of proteins that contain a carboxylic acid group…

Q: Which one of the following would best allow us to conclude that the rate of chymotrypsin was…

A: Chymotrypsin is a serine protease enzyme that aids in digestion in several organisms including…

Q: Which of these situations would produce a Hill plot with a Hill coefficient less than 1? A purified…

A: A Hill plot is used to establish the cooperativity of ligand binding to a protein.NH (Hill…

Q: At pH-4, what is the net charge of the peptide GKPAD? O+0.5 O-1.0 Oo -0.5

A: The sequence of the given peptide is as follows: Gly-Lys-Pro-Ala-Asp The amino acids are composed of…

Q: Peptides can be separated according to their charges via an ion-exchange column. In a…

A: Ion exchange chromatography is used to separate molecules based on the charge. Anion and cation…

Q: The shape of these peptide amphiphiles can be roughly. approximated by three cylindrical regions…

A: The peptide can have hydrophilic regions which means they are water-loving residues or regions that…

Q: A researcher is sequencing a peptide using Edman degradation. However upon examination of the…

A: Edman degradation is a 3-step procedure of the coupling of phenylisothiocyanate or PITC to the…

Q: For the binding of a ligand to a protein, what is the relationship between the Ka (association…

A: The ligand in protein-ligand binding is typically a molecule that generates a message by connecting…

Q: Which ligand-binding curve in the figure below would be consistent with the protein exhibiting a Ka…

A: Consider the interaction between a protein (P) and ligand (L) as shown below.Here, Ka is the…

Q: A researcher performed an SDS-PAGE experiment (with 2- mercaptoethanol) on a mixture containing 3…

A: G

Q: The following data describe the catalysis of cleavage of peptide bonds insmall peptides by the…

A: Elastase is a serine protease produced by the pancreas that catalyzes the cleavage of carboxyl…

Q: The following peptide that has been acetylated at the C-terminus: CH3C(O)-GEEGH-NH2 Plot the…

A: Titration is a very useful procedure to estimate the concentration of any unknown compound in a…

Q: What percentage of molecules of peptide GGGG has no ionized groups (e.g. has BOTH protonated…

A: Amino acids: An amino acid can function as both an acid and a base because of its structural…

Q: Please help me

A: Enzymes are proteins that enhance the rate of a biochemical reaction. They are sensitive to…

Q: Given below are sequences of amino acids present in an oligo-peptide chain. Count the overall charge…

A: Amino acids are the monomeric units that are used to form a peptide or protein and have chiral…

Q: Which of the following is incorrect about the peptide Arg-Asp-Cys-Tyr-Gln-Arg-Glu? O a. Its…

A: The peptide given is represented in single letter codes as: RDCYQRE Its structure is:

Q: When specific conditions are met, the creation of peptide bonds rather than the hydrolysis of…

A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…

Q: The following data describe the catalysis of cleavage of peptide bonds in small peptides by a…

A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…

Q: At a pH of 10, would you expect this peptide to be retained for a longer time within an anion…

A: Ion exchange chromatography separates molecules based on their charge difference. A cation exchange…

Q: A direct curren was passed through a solution containing valine, histidine, and aspartic acid a pH…

A: Amino acids are composed of carbon, nitrogen, hydrogen, and oxygen atoms. Each amino acid has a…

Q: Which way is more common practice to characterize the strength of a binding reaction between a…

A: Answer : the more common practice to characterize the strength of a binding reaction between a…

Q: The investigators reported that the sphingosine kinase enzyme attached to the DEAE column when Tris…

A: Buffers are composed of either a weak acid and its conjugate base or a weak base and its conjugate…

Q: 1. Refer to Model 2 for information relevant to this question a) A sample of the peptide Lys-Glu-Ser…

A: Detailed explanation: 1a) A sample of the peptide Lys-Glu-Ser has a net charge of zero between…

Q: A decapeptide was analyzed and the following information obtained. Determine the primary structure…

A: Proteins are polymers of amino acids. Amino acids are bound together by peptide bonds. An amino acid…

Q: at punctures the bacterial cell wall has just been recently isolated from the F the peptide reveals…

A: Trypsin cleave on the carboxy side of arginine and lysine residues. Chymotrypsin cleave on the…

Q: Determine the net charge of the following peptide at pH 10 given the respective pKa's. DDDMCRVNAY…

A: The peptide is the sequence of amino acids that are joined by the formation of peptide bond between…

Q: The function of the selectivity filter within the voltage-gated K* channel relies on interactions…

A: The KcsA channel is a type of ion channel protein found in the cell membranes of certain bacteria.…

Q: Sketch a plot that shows a typical binding curve for the interactions between a ligand, L (in terms…

A: Binding sites on proteins can be bound by a variety of molecules, called as ligands. Binding…

Q: The peptide below is placed into a buffer with a pH = 9.1 H₂N-CH-C- -NH–CH—CNHCHC NH–CH CH3 HỌ-CH,…

A: Electrophoresis means the migration of a charged particle under the influence of an applied electric…

Q: What is the isoelectric point of the following peptide molecule?…

A: The pH at which a specific molecule carries no net electrical charge is known as the isoelectric…

Q: Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At…

A: Isoelectric point: It is also known as pI; pH at which a peptide carries no net electrical charge or…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.