A 16 kb linear DNA is cut with EcoRI and BamHI. The EcoRI digest yields fragments of 2, 6, and 8 kb. The BamHI digest yield: fragments of 5 and11 kb. An EcoRI-BamHI double digest yields 2, 3, 5, and 6 kb fragments. The map of this DNA has 2 EcoRI sites and 2 Bam HI sites. 2 EcoRI sites and 1 BamHI site. 1 EcoRI site and 2 Bam HI sites. 3 BamHI sites and 2 ECORI sites. (E) 3 EcoRI sites and 2 BamHI sites.

Q: The following is the base sequence of DNA that codes for amino acids 506-510 of the protein that…

A: Introduction  Deoxyribonucleic acid is a polymer made of two polynucleotide chains that coil around…

Q: A piece of linear DNA is 700000 base pairs in length and is digested with a restriction enzyme that…

A: Linear DNA is found in prokaryotes, which is linear in shape with two free ends. Restriction enzymes…

Q: In the next gel, in lane I is the marker. Estimate the sizes of DNA fragments (in base pairs, bp)

A: Electrophoresis is the migration of charged particles under the influence of electric current.…

Q: A cloned fragment of DNA was sequenced by using the dideoxy method. A part of the autoradiogram of…

A: Sanger sequencing, often called chain-termination sequencing, is a DNA sequencing technology…

Q: DNA polymerases have exonuclease activity that hydrolyzes DNA in the 3′ to 5′ direction. When an…

A: DNA POLYMERASE: DNA polymerase is an enzyme that plays a key role in the replication of DNA, the…

Q: This is a restriction map for the 250 base pair plasmid pSage. Restriction sites for the restriction…

A: Restrictions endonucleases are enzymes that cut down the DNA into fragments near or at the site of…

Q: Fill in the blank spaces by selecting the correct enzyme for each of the restriction enzyme…

A: DNA  is cleaved to form DNA fragments with the help of an enzyme known as the restriction enzyme.…

Q: When half of the DNA in the sample is double-stranded, that point is termed the half- reaction time…

A: Cot values give the renaturation index of the DNA strands. Cot 1/2 value defines that 50%…

Q: When circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed…

A: It is related to molecular biology in which we use restriction enzymes an important tool for

Q: During a typical gel electrophoresis set up (negative pole at the top), which DNA fragment would be…

A: Gel electrophoresis is the technique for the separation of DNA fragments on the basis of size and…

Q: The -10 and -35 sequences in E. coli DNA are referred to as untranslated region. T/F

A: Transcription is defined as the synthesis of mRNA from the template strand of DNA by the action of…

Q: A new restriction enzyme is discovered. It recognizes a 6 base pair pallindromic sequence. The first…

A: BASIC INFORMATION  RESTRICTION ENZYME  they are also known as molecular scissors it was in the year…

Q: Consider the following image of a stained agarose gel representing the separation of a plece of DNA…

A: Gel electrophoresis is a biochemical technique in which macromolecules such as DNA  can be separated…

Q: Order the steps required to sequence a region of DNA using dideoxy sequencing. Amplify the region of…

A: Step 1: The order of the steps required to sequence a region of DNA using dideoxy sequencing…

Q: About 60% of the base pairs in the human genome are AT. If the human genome has 3.2 billion base…

A: An enzyme produced from the bacteria which recognizes specific base sequence in DNA and cut the DNA…

Q: The original DNA template starts with T following the primer sequence if the smallest fragment…

A: Dideoxy nucleotides are similar to regular, or deoxy, nucleotides, but they lack a  3’ OH group.…

Q: You are asked to sequence a piece of DNA to determine if it is from a gene thought to be involved in…

A: DNA sequencing is an important technique used to determine the sequence of unknown fragments of DNA…

Q: Which of the following is/are true of DNA general recombination? The process involves invasion of a…

A: DNA recombination in general is a natural process that occurs in a wide range of organisms,…

Q: Write the 3' to 5' DNA bottom strand • Write the mRNA sequence of top strand Write the mRNA sequence…

A: In DNA   there are four nitrogen as base that is adenine, Thymine, guanine and cytosine is present.…

Q: What is the role of alcohol in extracting DNA?

A: DNA (Deoxy Ribonucelic Acid) extraction is carried out to perform experiments. The first…

Q: Given the Following DNA template, TAC   CGC   TCC   GCC  GTC   GAC   AAT   ACC   ACT, write out the…

A: The DNA template is used for the synthesis of mRNA molecules. mRNA molecule is used by the…

Q: A student wanted to load .75 ug dna on agarose gel and has 4x loading buffer for sample preparation.…

A: Agarose gel electrophoresis is a method used for the separation of DNA fragments which is stained by…

Q: When linear DNA is sequenced, the nucleotide base pairs are numbered from the start to finish. a DNA…

A: DNA is the genetic material present in all living cells. It carries information from one cell to…

Q: Write the nucleotide sequence, including the primer, in the 3'to 5' direction. 3'- -5'

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Q: Decide on two restriction sites that you can use to clone this into pL4440’s MCS. Identify their…

A: The recombinant DNA technology use plasmid to transfer foreign DNA into the host organism in which…

Q: You have the following DNA sequence: 5'GGT ACG TTG GGG CTC CAT3' This sequence is transcribed and…

A: “Since you have posted multiple questions, we will provide the solution only to the first question…

Q: between the two restriction sites, how would the banding pattern on the gel differ from the one you…

A: Restriction sites are the special palindromic sequences present in DNA. These sites are the location…

Q: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is…

A: Using specialized enzymes known as restriction endonucleases, restriction digestion is a laboratory…

Q: CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct?…

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Q: You are considering using restriction enzymes that recognizes 4-bp and 6-bp nucleotide sequences,…

A: From theory we know, the longer the restriction site recognized by a particular restriction…

Q: An 800 bp fragment is completely digested with a single restriction enzyme. Fragments of 50*, 150*,…

A: Restriction enzymes are those enzymes which recognize and cleave at a particular sequence. In this…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.