9. Recombinant DNA could be transformed into a cell through various methods such as: Transformation method Description Ligated product incubated with competent E. coli, heat-shock treatment. Cells incubated with the plasmid DNA are subjected to a high-voltage pulse. Transiently renders the bacterial membrane permeable to large molecules. The process of injection of foreign DNA into a cell using micropipette/micromanipulator.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
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