9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.65. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. 010 CH3 H₂O CH3-C-O-CH2-CH2-N-CH3 CH3 Acetylcholine CH3 HO-CH2-CH2-N-CH3 + CH3-C-0¯ + H+ CH3 Choline Acetate At the end of the incubation period, you measure the pH again and find that it has decreased to 6.87. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample.
9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.65. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. 010 CH3 H₂O CH3-C-O-CH2-CH2-N-CH3 CH3 Acetylcholine CH3 HO-CH2-CH2-N-CH3 + CH3-C-0¯ + H+ CH3 Choline Acetate At the end of the incubation period, you measure the pH again and find that it has decreased to 6.87. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample.
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Transcribed Image Text:9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL
sample of acetylcholine (a neurotransmitter) with an unknown concentration
and a pH of 7.65. You incubate this sample with the enzyme
acetylcholinesterase to convert all of the acetylcholine to choline and acetic
acid. The acetic acid dissociates to yield acetate and hydrogen ions.
010
CH3
H₂O
CH3-C-O-CH2-CH2-N-CH3
CH3
Acetylcholine
CH3
HO-CH2-CH2-N-CH3 + CH3-C-0¯ + H+
CH3
Choline
Acetate
At the end of the incubation period, you measure the pH again and find that it
has decreased to 6.87. Assuming there was no buffer in the assay mixture,
determine the number of nanomoles of acetylcholine in the original 15 mL
sample.
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