9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.65. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. 010 CH3 H₂O CH3-C-O-CH2-CH2-N-CH3 CH3 Acetylcholine CH3 HO-CH2-CH2-N-CH3 + CH3-C-0¯ + H+ CH3 Choline Acetate At the end of the incubation period, you measure the pH again and find that it has decreased to 6.87. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample.

Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 98QRT: The average normal concentration of Ca2+ in urine is 5.33 g/L. Calculate the concentration of...
Question
None
9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL
sample of acetylcholine (a neurotransmitter) with an unknown concentration
and a pH of 7.65. You incubate this sample with the enzyme
acetylcholinesterase to convert all of the acetylcholine to choline and acetic
acid. The acetic acid dissociates to yield acetate and hydrogen ions.
010
CH3
H₂O
CH3-C-O-CH2-CH2-N-CH3
CH3
Acetylcholine
CH3
HO-CH2-CH2-N-CH3 + CH3-C-0¯ + H+
CH3
Choline
Acetate
At the end of the incubation period, you measure the pH again and find that it
has decreased to 6.87. Assuming there was no buffer in the assay mixture,
determine the number of nanomoles of acetylcholine in the original 15 mL
sample.
Transcribed Image Text:9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.65. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. 010 CH3 H₂O CH3-C-O-CH2-CH2-N-CH3 CH3 Acetylcholine CH3 HO-CH2-CH2-N-CH3 + CH3-C-0¯ + H+ CH3 Choline Acetate At the end of the incubation period, you measure the pH again and find that it has decreased to 6.87. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample.
Expert Solution
steps

Step by step

Solved in 2 steps with 1 images

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry: The Molecular Science
Chemistry: The Molecular Science
Chemistry
ISBN:
9781285199047
Author:
John W. Moore, Conrad L. Stanitski
Publisher:
Cengage Learning
Introductory Chemistry: A Foundation
Introductory Chemistry: A Foundation
Chemistry
ISBN:
9781337399425
Author:
Steven S. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
General Chemistry - Standalone book (MindTap Cour…
General Chemistry - Standalone book (MindTap Cour…
Chemistry
ISBN:
9781305580343
Author:
Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:
Cengage Learning
Introductory Chemistry For Today
Introductory Chemistry For Today
Chemistry
ISBN:
9781285644561
Author:
Seager
Publisher:
Cengage
Chemistry for Today: General, Organic, and Bioche…
Chemistry for Today: General, Organic, and Bioche…
Chemistry
ISBN:
9781305960060
Author:
Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:
Cengage Learning
Chemistry for Engineering Students
Chemistry for Engineering Students
Chemistry
ISBN:
9781285199023
Author:
Lawrence S. Brown, Tom Holme
Publisher:
Cengage Learning