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8. Glucose-6-phosphate dehydrogenase catalyzes the same reaction shown in problem 7 in yeast. The KM for yeast glucose-6-phosphate is 2.0 × 10-5 M and the KM for NADP+ is 2.0 x 10-6 M. The yeast enzyme can be inhibited by a number of cellular agents whose K₁ values are listed in the following table. Inhibitor Inorganic phosphate Glucosamine-6-phosphate NADPH K₁ (M) 1.0 x 10-1 7.2 × 10-4 2.7 x 10-5 a. Which is the most effective inhibitor? Explain. b. b. Which inhibitor(s) is (are) likely to be completely ineffective under normal cellular conditions? Explain.

8. Glucose-6-phosphate dehydrogenase catalyzes the same reaction shown in problem 7 in yeast. The KM for yeast glucose-6-phosphate is 2.0 × 10-5 M and the KM for NADP+ is 2.0 x 10-6 M. The yeast enzyme can be inhibited by a number of cellular agents whose K₁ values are listed in the following table. Inhibitor Inorganic phosphate Glucosamine-6-phosphate NADPH K₁ (M) 1.0 x 10-1 7.2 × 10-4 2.7 x 10-5 a. Which is the most effective inhibitor? Explain. b. b. Which inhibitor(s) is (are) likely to be completely ineffective under normal cellular conditions? Explain.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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7. Glucose-6-phosphate dehydrogenase catalyzes the reaction shown below. Kinetic data for the enzyme isolated from the thermophilic bacterium T. maritima are presented in the following table. CH₂OPO- CH₂OPO NADP+ FEB 25 HH HO OH H Н Glucose-6-phosphate Substrate H HO NADP+ NAD+ HO Glucose-6-phosphate NADPH + H+ glucose-6- phosphate dehydrogenase tv 12.0 H ОН W Aa H 6-Phosphoglucono-8-lactone KM (mm) Vmax (U • mg¯¹) 0.15 0.03 20 20 H OH H 9 a. NADPH inhibits glucose-6-phosphate dehydrogenase. What kind of inhibitor is HO BOA P noozinic oniOSONATE LUX TU - IGICosamune-O-osonate /ZXTU - 4 NADPH ZX 10- daloldadobolele X O of 1208 words
Transcribed Image Text:7. Glucose-6-phosphate dehydrogenase catalyzes the reaction shown below. Kinetic data for the enzyme isolated from the thermophilic bacterium T. maritima are presented in the following table. CH₂OPO- CH₂OPO NADP+ FEB 25 HH HO OH H Н Glucose-6-phosphate Substrate H HO NADP+ NAD+ HO Glucose-6-phosphate NADPH + H+ glucose-6- phosphate dehydrogenase tv 12.0 H ОН W Aa H 6-Phosphoglucono-8-lactone KM (mm) Vmax (U • mg¯¹) 0.15 0.03 20 20 H OH H 9 a. NADPH inhibits glucose-6-phosphate dehydrogenase. What kind of inhibitor is HO BOA P noozinic oniOSONATE LUX TU - IGICosamune-O-osonate /ZXTU - 4 NADPH ZX 10- daloldadobolele X O of 1208 words
8. Glucose-6-phosphate dehydrogenase catalyzes the same reaction shown in problem 7 in yeast. The KM for yeast glucose-6-phosphate is 2.0 × 10-5 M and the KM for NADP+ is 2.0 x 10-6 M. The yeast enzyme can be inhibited by a number of cellular agents whose K₁ values are listed in the following table. Inhibitor Inorganic phosphate Glucosamine-6-phosphate NADPH K₁ (M) 1.0 x 10 -1 7.2 × 10−4 2.7 x 10-5 a. Which is the most effective inhibitor? Explain. b. b. Which inhibitor(s) is (are) likely to be completely ineffective under normal cellular conditions? Explain.
Transcribed Image Text:8. Glucose-6-phosphate dehydrogenase catalyzes the same reaction shown in problem 7 in yeast. The KM for yeast glucose-6-phosphate is 2.0 × 10-5 M and the KM for NADP+ is 2.0 x 10-6 M. The yeast enzyme can be inhibited by a number of cellular agents whose K₁ values are listed in the following table. Inhibitor Inorganic phosphate Glucosamine-6-phosphate NADPH K₁ (M) 1.0 x 10 -1 7.2 × 10−4 2.7 x 10-5 a. Which is the most effective inhibitor? Explain. b. b. Which inhibitor(s) is (are) likely to be completely ineffective under normal cellular conditions? Explain.
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