6.) One way to determine the amount of nitrogen in a sample is through a method called Kjeldahl Method. In this method, the nitrogen in the sample is converted first to ammonium ions (N ? NH4+). The ammonium ions are then converted to ammonia by making the solution basic with the use of sodium hydroxide (NH4+ ? NH3). The ammonia produced is reacted with hydrochloric acid (NH3 +HCl ? NH4Cl). The unreacted hydrochloric acid is then titrated with standard sodium hydroxide solution. Using the method outlined, a 1-mL fish sample was analyzed. The liberated ammonia was collected in 100 mL of 0.0503 M HCl. The unreacted HCl then required 28.3 mL of 0.124 M NaOH for titration. Calculate the nitrogen in the sample as mg N/mL.
6.) One way to determine the amount of nitrogen in a sample is through a method called Kjeldahl Method. In this method, the nitrogen in the sample is converted first to ammonium ions (N ? NH4+). The ammonium ions are then converted to ammonia by making the solution basic with the use of sodium hydroxide (NH4+ ? NH3). The ammonia produced is reacted with hydrochloric acid (NH3 +HCl ? NH4Cl). The unreacted hydrochloric acid is then titrated with standard sodium hydroxide solution. Using the method outlined, a 1-mL fish sample was analyzed. The liberated ammonia was collected in 100 mL of 0.0503 M HCl. The unreacted HCl then required 28.3 mL of 0.124 M NaOH for titration. Calculate the nitrogen in the sample as mg N/mL.
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