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6. Calculate the average from the 2 experiments for each time point. Plot your data on a graph and calculate the rate (absorbance units minute ¹). Rate of the reaction based on your plotted values:

6. Calculate the average from the 2 experiments for each time point. Plot your data on a graph and calculate the rate (absorbance units minute ¹). Rate of the reaction based on your plotted values:

Chemistry: The Molecular Science
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter4: Energy And Chemical Reactions
Section: Chapter Questions
Problem 120QRT
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Time (Minutes) 0.5 1.0 1.5 200 205 3.0 3.5 4.0 4.5 6 5.0 Average 0.1045 001745 0.2495 0.3255 0.4005 0.4695 0.54 0.6035 0.6595 0.712
Transcribed Image Text:Time (Minutes) 0.5 1.0 1.5 200 205 3.0 3.5 4.0 4.5 6 5.0 Average 0.1045 001745 0.2495 0.3255 0.4005 0.4695 0.54 0.6035 0.6595 0.712
Apparatus: ●●●● ●●● P1000 Gilson and blue gilson tips 3 disposable cuvettes Spectrophotometer parafilm stop watch tissue Materials and Methods Chemicals: These chemicals are toxic, gloves and eye protection must be worn. All spills must be reported to the lecturer or a demonstrator and cleaned up immediately. Students must wash their hands before the laboratory is left. 50 mM sodium phosphate buffer, pH 7.5 (20ml) Distilled water 2 mM 2-nitrophenyl-ß-D-galactopyranoside (ONP-Gal) (5ml) 2 mM 2-nitrophenyl-ß-glucopyranoside (ONP-Glu) (5ml) 2 units.ml-¹ B-galactosidase (from E. coli) (10ml) Method: 1. Check that the spectrophotometer is on and set at 440 nm. If it is not set up correctly ASK for help. 3. In the second cuvette add: beslist elven 2. Fill the first cuvette with 3 ml distilled water and zero the spectrophotometer by placing the cuvette in the machine in the correct orientation and pressing the BLUE button. If you follow the instructions below you will not need to repeat this. 1 ml 50 mM sodium phosphate buffer, pH 7.5 1 ml Distilled water 0.5 ml 2-nitrophenyl-ß-D-galactopyranoside (ONP-Gal) <pause here before moving to next step> add 0.5ml ß-galactosidase to the cuvette WHEN YOU ARE READY TO START THE CLOCK Mix by covering the top of the cuvette with parafilm, ensure the outside of the cuvette is dry and place in the correct orientation (bevelled sides to the left and right) in the spectrophotometer. Press the GREEN button to start measuring the absorbance. 4. Take your first absorbance reading at 30 seconds and continue taking readings every 30 seconds for 5 minutes, record the reading in the form of a table (for table, see next page). 5. REPEAT the experiment starting at step 3. 6. Calculate the average from the 2 experiments for each time point. Plot your data on a graph and calculate the rate (absorbance units minute). Rate of the reaction based on your plotted values:
Transcribed Image Text:Apparatus: ●●●● ●●● P1000 Gilson and blue gilson tips 3 disposable cuvettes Spectrophotometer parafilm stop watch tissue Materials and Methods Chemicals: These chemicals are toxic, gloves and eye protection must be worn. All spills must be reported to the lecturer or a demonstrator and cleaned up immediately. Students must wash their hands before the laboratory is left. 50 mM sodium phosphate buffer, pH 7.5 (20ml) Distilled water 2 mM 2-nitrophenyl-ß-D-galactopyranoside (ONP-Gal) (5ml) 2 mM 2-nitrophenyl-ß-glucopyranoside (ONP-Glu) (5ml) 2 units.ml-¹ B-galactosidase (from E. coli) (10ml) Method: 1. Check that the spectrophotometer is on and set at 440 nm. If it is not set up correctly ASK for help. 3. In the second cuvette add: beslist elven 2. Fill the first cuvette with 3 ml distilled water and zero the spectrophotometer by placing the cuvette in the machine in the correct orientation and pressing the BLUE button. If you follow the instructions below you will not need to repeat this. 1 ml 50 mM sodium phosphate buffer, pH 7.5 1 ml Distilled water 0.5 ml 2-nitrophenyl-ß-D-galactopyranoside (ONP-Gal) <pause here before moving to next step> add 0.5ml ß-galactosidase to the cuvette WHEN YOU ARE READY TO START THE CLOCK Mix by covering the top of the cuvette with parafilm, ensure the outside of the cuvette is dry and place in the correct orientation (bevelled sides to the left and right) in the spectrophotometer. Press the GREEN button to start measuring the absorbance. 4. Take your first absorbance reading at 30 seconds and continue taking readings every 30 seconds for 5 minutes, record the reading in the form of a table (for table, see next page). 5. REPEAT the experiment starting at step 3. 6. Calculate the average from the 2 experiments for each time point. Plot your data on a graph and calculate the rate (absorbance units minute). Rate of the reaction based on your plotted values:
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