7₂226-27مارهYkma fumaxb=um max=1.4673kom=<=0,6815 mM minThe kinetic data in the following table were obtained for thereaction of carbon dioxide and water to produce bicarbonate andhydrogen ion catalyzed by carbonic anhydrase:CO2 + H2O - HCO3 + H+[H. De Voe and G. B. Distiakowsky, J. Am. Chem. Soc. 83, 274(1961)].km=aVmax=-CO₂ Concentration, mmol/L1.252.55.020.0From these data, use a Lineweaver-Burk plot to determine K andVmax for the reaction.=961730.41/Velocity (sec/M)36 x 10³20 x 10³12 x 1036 x 10³31 You do an enzyme kinetic experiment and calculate a Vmax of 1If each assay used 0.1 mL of arμmol of product per minute.enzyme solution that had a concentration of 02 mg/mL, whatthe turnover number if the enzyme had a molecular weight

According to Lineweaver-Burk plot- 1 / Velocity = Km / (Vmax* Concentration) + 1/Vmax Thus, on…

6-27 3.6815 mMmin The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase: CO2 + H2O HCO3 + H+ [H. De Voe and G. B. Distiakowsky, J. Am. Chem. Soc. 83, 274 (1961)]. From these data, use a Lineweaver-Burk plot to determine K and Vmax for the reaction. CO₂ Concentration, mmol/L 1.25 2.5 5.0 20.0 1/Velocity (sec/M) 36 x 103 20 x 10³ 12 x 103 6 x 10³

6-27 3.6815 mMmin The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase: CO2 + H2O HCO3 + H+ [H. De Voe and G. B. Distiakowsky, J. Am. Chem. Soc. 83, 274 (1961)]. From these data, use a Lineweaver-Burk plot to determine K and Vmax for the reaction. CO₂ Concentration, mmol/L 1.25 2.5 5.0 20.0 1/Velocity (sec/M) 36 x 103 20 x 10³ 12 x 103 6 x 10³

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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