5.2 5.3 Briefly discuss the importance of sample preparation in a modern-day laboratory. Using your understanding of separation science, how has sample preparation taken advantage of the theory of separation? The van Deemter equation relates the resolving power (HETP, height equivalent to a theoretical plate) of a chromatographic column to the various flow and kinetic parameters which cause peak broadening. H = A + B/u +Csu (a) Identify which term in the equation represents Eddy diffusion. (b) Explain how it contributes to peak broadening.
5.2 5.3 Briefly discuss the importance of sample preparation in a modern-day laboratory. Using your understanding of separation science, how has sample preparation taken advantage of the theory of separation? The van Deemter equation relates the resolving power (HETP, height equivalent to a theoretical plate) of a chromatographic column to the various flow and kinetic parameters which cause peak broadening. H = A + B/u +Csu (a) Identify which term in the equation represents Eddy diffusion. (b) Explain how it contributes to peak broadening.
Chapter14: Chromatography
Section: Chapter Questions
Problem 8P
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Question
![5.2
5.3
Briefly discuss the importance of sample preparation in a modern-day laboratory. Using your
understanding of separation science, how has sample preparation taken advantage of the theory
of separation?
The van Deemter equation relates the resolving power (HETP, height equivalent to a theoretical
plate) of a chromatographic column to the various flow and kinetic parameters which cause peak
broadening.
H = A + B/u +Csu
(a) Identify which term in the equation represents Eddy diffusion.
(b) Explain how it contributes to peak broadening.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4f7c5f7e-89d1-4748-8cce-53cd32114987%2Fb45ed9d9-8ddd-4b01-aea7-78c8461a9b05%2Fo1qea7_processed.jpeg&w=3840&q=75)
Transcribed Image Text:5.2
5.3
Briefly discuss the importance of sample preparation in a modern-day laboratory. Using your
understanding of separation science, how has sample preparation taken advantage of the theory
of separation?
The van Deemter equation relates the resolving power (HETP, height equivalent to a theoretical
plate) of a chromatographic column to the various flow and kinetic parameters which cause peak
broadening.
H = A + B/u +Csu
(a) Identify which term in the equation represents Eddy diffusion.
(b) Explain how it contributes to peak broadening.
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